Auxin-mediated Aux/IAA-ARF-HB signaling cascade regulates secondary xylem development in Populus

NWIPB OpenIR

	Auxin-mediated Aux/IAA-ARF-HB signaling cascade regulates secondary xylem development in Populus
	Xu, Changzheng; Shen, Yun; He, Fu; Fu, Xiaokang; Yu, Hong; Lu, Wanxiang; Li, Yongli; Li, Chaofeng; Fan, Di; Wang, Hua Cassan; Luo, Keming
	2019
发表期刊	NEW PHYTOLOGIST
卷号	222 期号:2
摘要	Wood development is strictly regulated by various phytohormones and auxin plays a central regulatory role in this process. However, how the auxin signaling is transducted in developing secondary xylem during wood formation in tree species remains unclear. Here, we identified an Aux/INDOLE-3-ACETIC ACID 9 (IAA9)-AUXIN RESPONSE FACTOR 5 (ARF5) module in Populus tomentosa as a key mediator of auxin signaling to control early developing xylem development. PtoIAA9, a canonical Aux/IAA gene, is predominantly expressed in vascular cambium and developing secondary xylem and induced by exogenous auxin. Overexpression of PtoIAA9m encoding a stabilized IAA9 protein significantly represses secondary xylem development in transgenic poplar. We further showed that PtoIAA9 interacts with PtoARF5 homologs via the C-terminal III/IV domains. The truncated PtoARF5.1 protein without the III/IV domains rescued defective phenotypes caused by PtoIAA9m. Expression analysis showed that the PtoIAA9-PtoARF5 module regulated the expression of genes associated with secondary vascular development in PtoIAA9m- and PtoARF5.1-overexpressing plants. Furthermore, PtoARF5.1 could bind to the promoters of two Class III homeodomain-leucine zipper (HD-ZIP III) genes, PtoHB7 and PtoHB8, to modulate secondary xylem formation. Taken together, our results suggest that the Aux/IAA9-ARF5 module is required for auxin signaling to regulate wood formation via orchestrating the expression of HD-ZIP III transcription factors in poplar.
关键词	ARF5 Aux/IAA9 auxin HD-ZIP III transcription factors Populus xylem development
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59822
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xu, Changzheng,Shen, Yun,He, Fu,et al. Auxin-mediated Aux/IAA-ARF-HB signaling cascade regulates secondary xylem development in Populus[J]. NEW PHYTOLOGIST,2019,222(2).
APA	Xu, Changzheng.,Shen, Yun.,He, Fu.,Fu, Xiaokang.,Yu, Hong.,...&Luo, Keming.(2019).Auxin-mediated Aux/IAA-ARF-HB signaling cascade regulates secondary xylem development in Populus.NEW PHYTOLOGIST,222(2).
MLA	Xu, Changzheng,et al."Auxin-mediated Aux/IAA-ARF-HB signaling cascade regulates secondary xylem development in Populus".NEW PHYTOLOGIST 222.2(2019).