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A convenient fluorescent assay for quinolones based on their inhibition towards the oxidase-like activity of Cu2+
Zhu, Shuyun; Cao, Han; Yan, Xiaolu; Sun, Jing; Qiu, Jiayi; Qu, Xiaoqing; Zuo, Ya-Nan; Wang, Xiao; Zhao, Xian-En
2019
Source PublicationNEW JOURNAL OF CHEMISTRY
Volume43Issue:9
AbstractIn this work, we proposed a convenient fluorescent assay for the detection of quinolones based on their inhibition towards the oxidation of o-phenylenediamine (OPD) by copper ions (Cu2+). Free Cu2+ can oxidize OPD into fluorescent 2,3-diaminophenzine followed by the formation of copper nanoparticles (CuNPs), which would further catalyze the oxidation of OPD. When quinolones were added, their strong binding affinity to Cu2+ inhibited the oxidase-like activity of Cu2+. So, the fluorescence intensity of the Cu2+-OPD system decreased obviously. The determination of quinolones can be achieved by using this fluorimetric assay without the use of complicated instruments and protocols. As demonstrated, this assay offered a highly sensitive readout for quinolones including nalidixic acid, cinoxacin, ciprofloxacin, and moxifloxacin in the linear ranges of 1.0-100 nM, 0.5-100 nM, 1.0-100 nM, and 0.1-100 nM, respectively. In addition, the developed assay was successfully applied to the direct analysis of quinolones in real amples.
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/59835
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Zhu, Shuyun,Cao, Han,Yan, Xiaolu,et al. A convenient fluorescent assay for quinolones based on their inhibition towards the oxidase-like activity of Cu2+[J]. NEW JOURNAL OF CHEMISTRY,2019,43(9).
APA Zhu, Shuyun.,Cao, Han.,Yan, Xiaolu.,Sun, Jing.,Qiu, Jiayi.,...&Zhao, Xian-En.(2019).A convenient fluorescent assay for quinolones based on their inhibition towards the oxidase-like activity of Cu2+.NEW JOURNAL OF CHEMISTRY,43(9).
MLA Zhu, Shuyun,et al."A convenient fluorescent assay for quinolones based on their inhibition towards the oxidase-like activity of Cu2+".NEW JOURNAL OF CHEMISTRY 43.9(2019).
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