NWIPB OpenIR
Transcriptome Analysis Clarified Genes Involved in Betalain Biosynthesis in the Fruit of Red Pitayas (Hylocereus costaricensis)
Xi, Xingyuan; Zong, Yuan; Li, Shiming; Cao, Dong; Sun, Xuemei; Liu, Baolong
2019
Source PublicationMOLECULES
Volume24Issue:3
AbstractThe red flesh trait gives red pitayas more healthful components and a higher price, while the genetic mechanism behind this trait is unknown. In this manuscript, transcriptome analysis was employed to discover the genetic differences between white and red flesh in pitayas. A total of 27.99 Gb clean data were obtained for four samples. Unigenes, 79,049 in number, were generated with an average length of 1333 bp, and 52,618 Unigenes were annotated. Compared with white flesh, the expression of 10,215 Unigenes was up-regulated, and 4853 Unigenes were down-regulated in red flesh. The metabolic pathways accounted for 64.6% of all differentially expressed Unigenes in KEGG pathways. The group with high betalain content in red flesh and all structural genes, related to betalain biosynthesis, had a higher expression in red flesh than white flesh. The expression of the key gene, tyrosine hydroxylase CYP76AD1, was up-regulated 245.08 times, while 4,5-DOPA dioxygenase DODA was up-regulated 6.46 times. Moreover, the special isomers CYP76AD1 and DODA were only expressed in red flesh. The competitive anthocyanin biosynthesis pathway had a lower expression in red flesh. Two MYB transcription factors were of the same branch as BvMYB1, regulating betalain biosynthesis in beet, and those transcription factors had expression differences in two kinds of pitayas, which indicated that they should be candidate genes controlling betalain accumulation in red pitayas. This research would benefit from identifying the major gene controlling red flesh trait and breed new cultivars with the red flesh trait. Future research should aim to prove the role of each candidate gene in betalain biosynthesis in red pitayas.
Keywordpitayas red flesh transcriptome analysis betalain biosynthesis
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/59865
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Xi, Xingyuan,Zong, Yuan,Li, Shiming,et al. Transcriptome Analysis Clarified Genes Involved in Betalain Biosynthesis in the Fruit of Red Pitayas (Hylocereus costaricensis)[J]. MOLECULES,2019,24(3).
APA Xi, Xingyuan,Zong, Yuan,Li, Shiming,Cao, Dong,Sun, Xuemei,&Liu, Baolong.(2019).Transcriptome Analysis Clarified Genes Involved in Betalain Biosynthesis in the Fruit of Red Pitayas (Hylocereus costaricensis).MOLECULES,24(3).
MLA Xi, Xingyuan,et al."Transcriptome Analysis Clarified Genes Involved in Betalain Biosynthesis in the Fruit of Red Pitayas (Hylocereus costaricensis)".MOLECULES 24.3(2019).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xi, Xingyuan]'s Articles
[Zong, Yuan]'s Articles
[Li, Shiming]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xi, Xingyuan]'s Articles
[Zong, Yuan]'s Articles
[Li, Shiming]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xi, Xingyuan]'s Articles
[Zong, Yuan]'s Articles
[Li, Shiming]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.