An effective method based on medium-pressure liquid chromatography and recycling high-speed counter-current chromatography for enrichment and separation of three minor components with similar polarity from Dracocephalum tanguticum

NWIPB OpenIR

	An effective method based on medium-pressure liquid chromatography and recycling high-speed counter-current chromatography for enrichment and separation of three minor components with similar polarity from Dracocephalum tanguticum
	Yang, Xue; Wang, Nana; Shen, Cheng; Li, Hongmei; Zhao, Jingyang; Chen, Tao; Li, Yulin
	2019
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	42 期号:3
摘要	The separation of minor compounds, especially those with similar polarities from a complex sample, remains challenging. In the proposed study, an effective method based on medium-pressure liquid chromatography and recycling high-speed counter-current chromatography was developed for the enrichment and separation of three minor components from Dracocephalum tanguticum. The crude extract was directly introduced to medium-pressure liquid chromatography for the enrichment of the three minor components. Based on high-performance liquid chromatography analysis, the total content of these three compounds increased from 0.48% in the crude extract to 85.3% in the medium-pressure liquid chromatography fraction. In addition, high-speed counter-current chromatography was employed to separate the enriched compounds using the solvent system hexane/ethyl acetate/methanol/water (1.18: 8.82: 1.18: 8.82, v/v/v/v). As a result, compound 3 and a mixture of compounds 1 and 2 were obtained. In order to improve the resolution of compounds 1 and 2 while saving separation time, a recycling and heart-cut mode was used. Finally, compounds 1 and 2 were obtained after five cycles. These compounds were identified as 3-phenylethyl beta-D-glucopyranoside (1), tazettoside E (2), and cirsiliol-4'-glucoside (3). Compounds 1 and 2 were primarily separated from D. tanguticum. Moreover, the developed method provided a reference for the separation of minor components from the complex sample.
关键词	Dracocephalum tanguticum heart-cut high-speed counter-current chromatography minor components recycling separation
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59867
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Xue,Wang, Nana,Shen, Cheng,et al. An effective method based on medium-pressure liquid chromatography and recycling high-speed counter-current chromatography for enrichment and separation of three minor components with similar polarity from Dracocephalum tanguticum[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(3).
APA	Yang, Xue.,Wang, Nana.,Shen, Cheng.,Li, Hongmei.,Zhao, Jingyang.,...&Li, Yulin.(2019).An effective method based on medium-pressure liquid chromatography and recycling high-speed counter-current chromatography for enrichment and separation of three minor components with similar polarity from Dracocephalum tanguticum.JOURNAL OF SEPARATION SCIENCE,42(3).
MLA	Yang, Xue,et al."An effective method based on medium-pressure liquid chromatography and recycling high-speed counter-current chromatography for enrichment and separation of three minor components with similar polarity from Dracocephalum tanguticum".JOURNAL OF SEPARATION SCIENCE 42.3(2019).