Highly fluorescent N-doped carbon dots with two-photon emission for ultrasensitive detection of tumor marker and visual monitor anticancer drug loading and delivery

NWIPB OpenIR

	Highly fluorescent N-doped carbon dots with two-photon emission for ultrasensitive detection of tumor marker and visual monitor anticancer drug loading and delivery
	Gong, Peiwei; Sun, Lu; Wang, Fei; Liu, Xicheng; Yan, Zhengquan; Wang, Mengzhen; Zhang, Lei; Tian, Zhenzhen; Liu, Zhe; You, Jinmao
	2019
发表期刊	CHEMICAL ENGINEERING JOURNAL
卷号	356
摘要	Early diagnosis and therapy are practically important to effectively prevent cancer and improve patients' life quality. Herein, a simple and sensitive two-photon imaging fluorescent nitrogen-doped carbon dots (N-CDs) platform was constructed to detect beta-glucuronidase (beta G, a tumor-invasive biomarker) based on inner filter effect (IFE), to visually monitor anticancer drug loading by fluorescence resonance energy transfer (FRET), and to effectively treat cancer by chemotherapy. To build this sensing platform, novel N-CDs with strong green emission (Ca. QY= 45%) were synthesized by one-pot pyrolysis reaction, and specifically, careful structure design of the excitation bands of N-CDs well covered the absorption bands of p-nitrophenol (PNP, beta G catalytic product), making it ultra-sensitive towards the activity of beta G and showing good linear relationship from 1 to 15 U/L and exciting detection limit of 0.3 U/L (S/N=3). This sensing platform has been successfully employed for beta G sensing in both human serum and intracellular detection. Moreover, we also demonstrated the first application of N-CDs as a novel visual detection sensor to monitor the loading process of doxorubicin, and as a nanocarrier for cancer therapy. More importantly, the N-CDs also exhibited high quality single-and two-photon fluorescence in high depth (18 mu m), enabling the N-CDs to real-time monitor the endocytosis, intracellular distribution and release of drugs, indicating significant potential for biomedicine and bioimaging applications.
关键词	Carbon dots Inner filter effect beta-Glucuronidase Nanocarrier Chemotherapy
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59874
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Gong, Peiwei,Sun, Lu,Wang, Fei,et al. Highly fluorescent N-doped carbon dots with two-photon emission for ultrasensitive detection of tumor marker and visual monitor anticancer drug loading and delivery[J]. CHEMICAL ENGINEERING JOURNAL,2019,356.
APA	Gong, Peiwei.,Sun, Lu.,Wang, Fei.,Liu, Xicheng.,Yan, Zhengquan.,...&You, Jinmao.(2019).Highly fluorescent N-doped carbon dots with two-photon emission for ultrasensitive detection of tumor marker and visual monitor anticancer drug loading and delivery.CHEMICAL ENGINEERING JOURNAL,356.
MLA	Gong, Peiwei,et al."Highly fluorescent N-doped carbon dots with two-photon emission for ultrasensitive detection of tumor marker and visual monitor anticancer drug loading and delivery".CHEMICAL ENGINEERING JOURNAL 356(2019).