NWIPB OpenIR
Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin
Xue, Fangfang; Qu, Fei; Han, Wenli; Xia, Lian; You, Jinmao
2019
发表期刊ANALYTICA CHIMICA ACTA
卷号1046
摘要Metal nanoclusters protected by glutathione (GSH) have attracted a wide attention due to the unique aggregation-induced emission (AIE) feature. However, the "trigger" effects of ethanol, temperature, pH values, and metal ions may restrict the application of these particles. In this work, the amino modified silicon nanoparticles (SiNPs) and GSH-capped gold nanoclusters (GSH-AuNCs) can self-assemble into well-defined spherical particles due to the electrostatic interaction. As a result, the unique aggregation-induced emission enhancement (AIEE) of GSH-AuNCs arises at 570 nm, and the SiNPs keep their own blue fluorescence at 450 nm, so a novel nanohybrid probe (SiNPs@GSH-AuNCs) with dual-emission property has been constructed. When protamine is added to SiNPs@GSH-AuNCs, the cationic protamine can compete with SiNPs and absorb onto the surface of GSH-AuNCs, which inhibits the self-assembly and leads to the fluorescence quenching of GSH-AuNCs; while trypsin can catalyze the hydrolysis of protamine, the self-assembly starts again, producing the AIEE recovery. In the whole process, the SiNPs act as an internal standard and their emission stays constant. By means of the fluorescence intensity ratios I-570/I-450, the linear range of protamine is from 0.15 to 3.00 mu gmL(-1) with the limit of detection (LOD) of 0.07 mu gmL(-1), and trypsin shows a linear response in the range from 10 to 100 ngmL(-1) with LOD of 4.50 ngmL(-1). Furthermore, this strategy exhibits good sensitivity and selectivity, and has been further validated by applying it for the determination of protamine and trypsin in serum samples. (C) 2018 Elsevier B.V. All rights reserved.
关键词Glutathione-capped gold nanoclusters Silicon nanoparticles Aggregation-induced emission Ratiometric detection Self-assembly
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/59877
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Xue, Fangfang,Qu, Fei,Han, Wenli,et al. Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin[J]. ANALYTICA CHIMICA ACTA,2019,1046.
APA Xue, Fangfang,Qu, Fei,Han, Wenli,Xia, Lian,&You, Jinmao.(2019).Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin.ANALYTICA CHIMICA ACTA,1046.
MLA Xue, Fangfang,et al."Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin".ANALYTICA CHIMICA ACTA 1046(2019).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xue, Fangfang]的文章
[Qu, Fei]的文章
[Han, Wenli]的文章
百度学术
百度学术中相似的文章
[Xue, Fangfang]的文章
[Qu, Fei]的文章
[Han, Wenli]的文章
必应学术
必应学术中相似的文章
[Xue, Fangfang]的文章
[Qu, Fei]的文章
[Han, Wenli]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。