Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii

NWIPB OpenIR

	Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
	Tian, Fei; Liu, Sijia; Shi, Jianquan; Qi, Hongfang; Zhao, Kai; Xie, Baosheng
	2019
发表期刊	BMC GENOMICS
卷号	20
摘要	BackgroundThe Tibetan highland fish, Gymnocypris przewalskii, migrates from Lake Qinghai to its spawning grounds every summer. This seasonal reproduction is critically regulated by intrinsic and extrinsic signals. However, the molecular mechanisms that process environmental oscillations to initiate the seasonal mating are largely unknown.ResultsA transcriptomic analysis was conducted on the brain and gonad of male and female G. przewalskii in reproductive and nonreproductive seasons. We obtained 2034, 760, 1158 and 17,856 differentially expressed genes between the reproductively active and dormant female brain, male brain, ovary and testis. Among these genes, DIO2 was upregulated in the reproductively active brain and gonad of both males and females. Neuroactive ligand-receptor genes were activated in male and female brain. Functional enrichment analysis suggested that retinol metabolism was uniquely stimulated in reproductively active males. Genes involved in GnRH signaling and sex hormone synthesis exhibited higher expression levels in brain and gonad during the reproductive season. A co-expression network classified all the genes into 9 modules. The network pinpointed CDC42 as the hub gene that connected the pathways in responsible for modulating reproduction in G. przewalskii. Meanwhile, the sex pheromone receptor gene prostaglandin receptor was identified to link to multiple endocrine receptors, such as GnRHR2 in the network.ConclusionsThe current study profiled transcriptomic variations between reproductively active and dormant fish, highlighting the potential regulatory mechanisms of seasonal reproduction in G. przewalskii. Our data suggested that the seasonal regulation of reproduction in G. przewalskii was controlled by the external stimulation of photoperiodic variations. The activated transcription of neuroendocrine and sex hormone synthesis genes contributed to seasonal reproduction regulation in G. przewalskii, which was presumably influenced by the increased day-length during the breeding season.
关键词	Seasonality Reproductive migration Neuroendocrine Day-length RNA-seq WGCNA Tibetan highland fish
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59878
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tian, Fei,Liu, Sijia,Shi, Jianquan,et al. Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii[J]. BMC GENOMICS,2019,20.
APA	Tian, Fei,Liu, Sijia,Shi, Jianquan,Qi, Hongfang,Zhao, Kai,&Xie, Baosheng.(2019).Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii.BMC GENOMICS,20.
MLA	Tian, Fei,et al."Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii".BMC GENOMICS 20(2019).