Hormesis of methylmercury-human serum albumin conjugate on N9 microglia via ERK/MAPKs and STAT3 signaling pathways

NWIPB OpenIR

	Hormesis of methylmercury-human serum albumin conjugate on N9 microglia via ERK/MAPKs and STAT3 signaling pathways
	Tan, Qiaozhu; Zhang, Ming; Geng, Lujing; Xia, Zhenghua; Lia, Cen; Usman, Muhammad; Du, Yuzhi; Wei, Lixin; Bi, Hongtao
	2019
发表期刊	TOXICOLOGY AND APPLIED PHARMACOLOGY
卷号	362
摘要	Methylmercury (MeHg+) is an extremely toxic organomercury cation that can induce severe neurological damage. Once it enters the body, methylmercury binds to amino acids or proteins containing free sulfhydryl groups. In particular, methylmercury is known to bind with human serum albumin (HSA) in human plasma; however, the effects of methylmercury-HSA conjugate (MeHg-HSA) on the central nervous system (CNS) are not fully understood. In the present study, we used the microglial cell line N9 as the target cells to evaluate the effect of MeHg-HSA on physiological function of the CNS preliminarily. The various factors in the cell culture were monitored by MTT assay, total lactate dehydrogenase assay, ELISA, qPCR, Western blot and flow cytometry techniques. The results showed that low-dose treatment with MeHg-HSA activated N9 cells, promoting cell proliferation and total cell number, enhancing NO and intracellular Ca2+ levels, and suppressing the release of TNF alpha and IL1 beta without cytotoxic effects; while high-dose MeHg-HSA exhibited cytotoxic effects on N9 cells, including promoting cell death and increasing the secretion of TNF alpha and IL1 beta. These results indicate that MeHg-HSA causes hormesis in microglia N9 cells. Furthermore, ERK/MAPKs and STAT3 signaling pathways related to the hormesis of MeHg-HSA on N9 cells. In addition, low dose of MeHg-HSA might be viewed as something very close to a lowest observed adverse effect level (LOAEL) for N9 cells. These findings will be useful for investigating the hormesis mechanism of MeHe+ and exploring the specific functions of MeHg-sulthydryl conjugates on the central nervous system.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59902
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tan, Qiaozhu,Zhang, Ming,Geng, Lujing,et al. Hormesis of methylmercury-human serum albumin conjugate on N9 microglia via ERK/MAPKs and STAT3 signaling pathways[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2019,362.
APA	Tan, Qiaozhu.,Zhang, Ming.,Geng, Lujing.,Xia, Zhenghua.,Lia, Cen.,...&Bi, Hongtao.(2019).Hormesis of methylmercury-human serum albumin conjugate on N9 microglia via ERK/MAPKs and STAT3 signaling pathways.TOXICOLOGY AND APPLIED PHARMACOLOGY,362.
MLA	Tan, Qiaozhu,et al."Hormesis of methylmercury-human serum albumin conjugate on N9 microglia via ERK/MAPKs and STAT3 signaling pathways".TOXICOLOGY AND APPLIED PHARMACOLOGY 362(2019).