Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell

NWIPB OpenIR

	Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell
	Huang, Yongzhen; Lai, Xinsheng; Hu, Linyong; Lei, Chuzhao; Lan, Xianyong; Zhang, Chunlei; Ma, Yun; Zheng, Li; Bai, Yue-Yu; Lin, Fengpeng; Chen, Hong
	2018
发表期刊	JOURNAL OF CELLULAR PHYSIOLOGY
卷号	233 期号:12
摘要	Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndrome. Previous studies suggested that DEC1 was involved in skeletal muscle development; however, its precise role in myoblast differentiation has not been determined. In the present study, we showed that DEC1 expressed ubiquitously in different bovine tissues and was down-regulated in differentiated bovine satellite cells. Expression of muscle specific transcription factors (Myf5, MyoD, MyoG, and MHC) was significantly down-regulated when DEC1 was over-expressed by both CoCl2-simulated hypoxia and Adenovirus-mediated transduction in bovine satellite cells. Consistent with that, promoter analyses via luciferase reporter assay also revealed that overexpression of bovine DEC1 could inhibit MyoG promoter activity. In conclusion, overexpression of DEC1 blocked myogenesis by inhibiting MyoG promoter activity in bovine. Our results provided a new mechanism for the muscle growth, which would contribute to increase cattle meat productivity.
关键词	cattle DEC1 MyoG satellite cell skeletal muscle
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59924
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Huang, Yongzhen,Lai, Xinsheng,Hu, Linyong,et al. Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2018,233(12).
APA	Huang, Yongzhen.,Lai, Xinsheng.,Hu, Linyong.,Lei, Chuzhao.,Lan, Xianyong.,...&Chen, Hong.(2018).Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell.JOURNAL OF CELLULAR PHYSIOLOGY,233(12).
MLA	Huang, Yongzhen,et al."Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell".JOURNAL OF CELLULAR PHYSIOLOGY 233.12(2018).