Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to alpha-glucosidase activity detection

NWIPB OpenIR

	Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to alpha-glucosidase activity detection
	Wu, Di; Hu, Na; Liu, Jichao; Fan, Guangsen; Li, Xiuting; Sun, Jing; Dai, Chunji; Suo, Yourui; Li, Guoliang; Wu, Yongning
	2018
发表期刊	TALANTA
卷号	190
摘要	Recently, exploitation of nanozymes for signal amplification has aroused extensive research interesting in the fields of analytical chemistry and nanoscience. Herein, we introduced a new sensing strategy based on the ascorbic acid (AA) precisely regulated Ag3PO4 nanozyme for signal amplification. AA can reduce the partial Ag+ on the surface of Ag3PO4 nano-particles to form Ag degrees particles and result in the formation of Ag degrees/Ag3PO4 heterostructure. Due to surface plasmon resonance (SPR), the Ag degrees particles in heterostructure can act as the co catalysts to enhance the electron-hole separation and the interfacial charge transfer, leading to a highly efficient oxidase mimicking activity for catalyzing the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) under visible light. Impressively, the activity enhancement of Ag3PO4 nano-peroxidase was linearly depended on the AA concentrations. Based on this feature, we employed it for multiple biological detections. For example, L-ascorbic acid-2-O-alpha-D-glucopyranosyl (AAG) was designed for alpha-glucosidase substrate, which can be hydrolyzed by alpha-glucosidase and lead to AA release. Further, the signal of a-glucosidase activity can be efficiently enlarged by Ag3PO4 nanozyme. Taking advantages of the powerful signal amplification by Ag3PO4 nanozymes, we developed the multiple sensing assays for monitoring AA in rat brain microdialysates, detection of alpha-glucosidase activity and its inhibitors (anti-diabetic drugs). The assay allowed the ultrasensitive colorimetric detection of alpha-aglucosidase inhibitor with an ultra-low detection limit of 10 nM, and a naked-eye detection of the inhibitor concentration as low as 1 mu M. The sensing strategy based on AA precisely regulated Ag3PO4 nano-peroxidase provides a promising candidate platform for highly stable and efficient cascaded signal amplification in biosensing field.
关键词	Nanozymes Photo-nanoperoxidase alpha-glucosidase Inhibitor screening
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59933
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wu, Di,Hu, Na,Liu, Jichao,et al. Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to alpha-glucosidase activity detection[J]. TALANTA,2018,190.
APA	Wu, Di.,Hu, Na.,Liu, Jichao.,Fan, Guangsen.,Li, Xiuting.,...&Wu, Yongning.(2018).Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to alpha-glucosidase activity detection.TALANTA,190.
MLA	Wu, Di,et al."Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to alpha-glucosidase activity detection".TALANTA 190(2018).