Delta FosB regulates rosiglitazone-induced milk fat synthesis and cell survival

NWIPB OpenIR

	Delta FosB regulates rosiglitazone-induced milk fat synthesis and cell survival
	Wei, Xuefeng; Li, Hui; Zhao, Guangwei; Yang, Jiameng; Li, Lihui; Huang, Yongzhen; Lan, Xianyong; Ma, Yun; Hu, Linyong; Zheng, Huiling; Chen, Hong
	2018
发表期刊	JOURNAL OF CELLULAR PHYSIOLOGY
卷号	233 期号:12
摘要	Rosiglitazone induces adipogenesis in adipocyte and regulates cell survival and differentiation in number of cell types. However, whether PPAR regulates the synthesis of milk fat and cell survival in goat mammary gland remains unknown. Rosiglitazone strongly enhanced cellular triacylglycerol content and accumulation of lipid droplet in goat mammary epithelial cells (GMEC). Furthermore, FosB decreased the expression of PPAR at both mRNA and protein levels, and rosiglitazone-induced milk fat synthesis was abolished by FosB overexpression. FosB reduced milk fat synthesis and enhanced saturated fatty acid concentration. Rosiglitazone increased the number of GMEC in G0/G1 phase and inhibited cell proliferation, and these effects were improved by overexpression of FosB. FosB was found to promote the expression of Bcl-2 and suppress the expression of Bax, and protected GMEC from apoptosis induced by rosiglitazone. Intracellular calcium trafficking assay revealed that rosiglitazone markedly increased intracellular calcium concentration. FosB protected GMEC from apoptosis induced by intracellular Ca2+ overload. FosB increased MMP-9 gelatinolytic activity. SB-3CT, an MMP-9 inhibitor, suppressed the expression of Bcl-2, and increased intracellular calcium levels, and this effect was abolished by FosB overexpression. SB-3CT induced GMEC apoptosis and this effect was inhibited by FosB overexpression. These findings suggest that FosB regulates rosiglitazone-induced milk fat synthesis and cell survival. Therefore, FosB may be an important checkpoint to control milk fat synthesis and cell apoptosis.
关键词	FosB apoptosis milk fat synthesis MMP-9 rosiglitazone
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59934
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wei, Xuefeng,Li, Hui,Zhao, Guangwei,et al. Delta FosB regulates rosiglitazone-induced milk fat synthesis and cell survival[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2018,233(12).
APA	Wei, Xuefeng.,Li, Hui.,Zhao, Guangwei.,Yang, Jiameng.,Li, Lihui.,...&Chen, Hong.(2018).Delta FosB regulates rosiglitazone-induced milk fat synthesis and cell survival.JOURNAL OF CELLULAR PHYSIOLOGY,233(12).
MLA	Wei, Xuefeng,et al."Delta FosB regulates rosiglitazone-induced milk fat synthesis and cell survival".JOURNAL OF CELLULAR PHYSIOLOGY 233.12(2018).