NWIPB OpenIR
Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep
Ma, Lin; Zhang, Meng; Jin, Yunyun; Erdenee, Sarantsetseg; Hu, Linyong; Chen, Hong; Cai, Yong; Lan, Xianyong
2018
发表期刊FRONTIERS IN GENETICS
卷号9
摘要The Lanzhou Fat-Tail sheep (LFTS, long fat-tailed sheep) is an endangered sheep breed in China with a fat tail compared to the traditional local varieties, Small Tail Han sheep (STHS, thin-tailed sheep) with a small tail, and Tibetan sheep (TS, short thin-tailed sheep) with a little tail. However, little is known regarding how tail fat deposition is regulated by long noncoding RNA (lncRNA). To evaluate the lncRNA and mRNA associated with tail fat deposition and development among these breeds, high-throughput RNA sequencing of three individuals each of LFTS, STHS, and TS were performed and analyzed in this study. RNA sequencing data from these three groups revealed 10 differentially expressed genes (DEGs) and 37 differentially expressed lncRNAs between the LFTS and STHS groups, 390 DEGs and 59 differentially expressed lncRNAs between the LFTS and TS groups, and 80 DEGs and 16 differentially expressed lncRNAs between the STHS and TS groups (p-value < 0.05 and fold change >= 2), respectively. Gene Ontology and pathway analysis of DEGs and target genes of differentially expressed lncRNAs revealed enrichment in fatty acid metabolism and fatty acid elongation-related pathways that contribute to fat deposition. Subsequently, the expression of 14 DEGs and 6 differentially expressed lncRNAs was validated by quantitative real-time PCR. Finally, two co-expression networks of differentially expressed mRNA and lncRNAs were constructed. The results suggested that some differentially expressed lncRNAs (TCONS_00372767, TCONS_00171926, TCONS_00054953, and TCONS_00373007) may play crucial roles as core lncRNAs in tail fat deposition processes. In summary, the present study extends the sheep tail fat lncRNA database and these differentially expressed mRNA and lncRNAs may provide novel candidate regulators for future genetic and molecular studies on tail fat deposition of sheep.
关键词sheep transcriptome fat deposition long non-coding RNA (lncRNA) fat tail
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/59974
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Ma, Lin,Zhang, Meng,Jin, Yunyun,et al. Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep[J]. FRONTIERS IN GENETICS,2018,9.
APA Ma, Lin.,Zhang, Meng.,Jin, Yunyun.,Erdenee, Sarantsetseg.,Hu, Linyong.,...&Lan, Xianyong.(2018).Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep.FRONTIERS IN GENETICS,9.
MLA Ma, Lin,et al."Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep".FRONTIERS IN GENETICS 9(2018).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma, Lin]的文章
[Zhang, Meng]的文章
[Jin, Yunyun]的文章
百度学术
百度学术中相似的文章
[Ma, Lin]的文章
[Zhang, Meng]的文章
[Jin, Yunyun]的文章
必应学术
必应学术中相似的文章
[Ma, Lin]的文章
[Zhang, Meng]的文章
[Jin, Yunyun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。