Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells

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	Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells
	Yuan, Zhen-zhen; Suo, You-rui; Hao, Xue-yan; Wang, Shu-lin; Li, Gang; Wang, Hong-lun
	2018
发表期刊	BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷号	41 期号:6
摘要	Six triterpenic acids were separated and purified from the ethyl acetate extractive fraction of ethanol extracts of Potentilla parvifolia Fiscx. using a variety of chromatographic methods. The neuroprotective effects of these triterpenoids were investigated in the present study, in which the okadaic acid induced neurotoxicity in human neuroblastoma SH-SY5Y cells were used as an Alzheimer's disease cell model in vitro. The cell model was established with all trans-retinoic acid (5pmol/L, 4d) and okadaic acid (40 nmol/L, 6h) treatments to induce tau phosphorylation and synaptic atrophy. Subsequently, the neuroprotective effects of these triterpenic acids were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3-6 had the better neuroprotective effects. Furthermore, we tested the level of mitochondrial reactive oxygen species and mitochondrial membrane potential of these compounds in SH-SY5Y cells by flow cytometry technology to investigate the potential neuroprotective mechanism of these compounds. All of the results indicated that maybe the mechanism of compounds 5 and 6 is to protect the cell from mitochondrial oxidative stress injuries.
关键词	Potentilla parvifolia triterpenic acid SH-SY5Y cell tau phosphorylation reactive oxygen species
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60015
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yuan, Zhen-zhen,Suo, You-rui,Hao, Xue-yan,et al. Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2018,41(6).
APA	Yuan, Zhen-zhen,Suo, You-rui,Hao, Xue-yan,Wang, Shu-lin,Li, Gang,&Wang, Hong-lun.(2018).Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells.BIOLOGICAL & PHARMACEUTICAL BULLETIN,41(6).
MLA	Yuan, Zhen-zhen,et al."Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells".BIOLOGICAL & PHARMACEUTICAL BULLETIN 41.6(2018).