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Separation of three polar compounds from Rheum tanguticum by high-speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system
Chen, Tao; Wang, Ping; Wang, Nana; Sun, Chongyang; Yang, Xue; Li, Hongmei; Zhou, Guoying; Li, Yulin
2018
Source PublicationJOURNAL OF SEPARATION SCIENCE
Volume41Issue:8
AbstractThe separation of polar compounds by high-speed countercurrent chromatography is still regarded as a challenge. In this study, an efficient strategy for the separation of three polar compounds from Rheum tanguticum has been successfully conducted by using high-speed countercurrent chromatography. X-5 macroporous resin chromatography was used for the fast enrichment of the target compounds. Then, the target fraction was directly introduced into high-speed countercurrent chromatography for separation using ethyl acetate/glacial acetic acid/water (100:1:100, v/v/v) as the solvent system. Consequently, three polar compounds including gallic acid, catechin, and gallic acid 4-O-beta-D-(6'-O-galloyl) glucoside were obtained with purities higher than 98%. The results showed glacial acetic acid could be such an appropriate regulator for the ethyl acetate/water system. This study provides a reference for the separation of polar compounds from natural products by high-speed countercurrent chromatography.
Keywordhigh-speed countercurrent chromatography polar compounds Rheum tanguticum traditional Chinese medicine
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/60019
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Chen, Tao,Wang, Ping,Wang, Nana,et al. Separation of three polar compounds from Rheum tanguticum by high-speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(8).
APA Chen, Tao.,Wang, Ping.,Wang, Nana.,Sun, Chongyang.,Yang, Xue.,...&Li, Yulin.(2018).Separation of three polar compounds from Rheum tanguticum by high-speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system.JOURNAL OF SEPARATION SCIENCE,41(8).
MLA Chen, Tao,et al."Separation of three polar compounds from Rheum tanguticum by high-speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system".JOURNAL OF SEPARATION SCIENCE 41.8(2018).
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