Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae

NWIPB OpenIR

	Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae
	Yan, Chongjia; Wang, Song; Wang, Jian; Li, Hui; Huang, Zhiwei; Sun, Jing; Peng, Min; Liu, Wenbin; Shi, Ping
	2018
发表期刊	MICROBIOLOGICAL RESEARCH
卷号	214
摘要	Clioquinol (CQ) has been used as a classical antimicrobial agent for many years. However, its mode of action is still unclear. In our study, the growth of Candida albicans and Saccharomyces cerevisiae was inhibited by CQ. It did not kill yeast cells, but shortened G1 phase and arrested cell cycle at G(2)/M phase. By using two-dimensional electrophoresis based proteomic approach, six proteins were found to be significantly affected by CQ. Among them, four (PDC1, ADH1, TDH3, IPP1) were up-regulated and the other two (TDH1 and PGK1) were downregulated. According to the Saccharomyces Genome Database (SGD), these proteins were involved in various biological processes including glycolytic fermentation, gluconeogenesis, glycolytic process, amino acid catabolism, redox reaction and reactive oxygen species metabolic process. It was noted that there was a link between TDH3 and cell cycle. The overexpression of TDH3 phenocopied CQ treatment and arrested the cell cycle at G(2)/M phase. RT-PCR analysis showed that the mRNA levels of CLN3 and CDC28, critical genes for passage through G1 phase, were up-regulated after the treatment of CQ as well as the overexpression of TDH3. It demonstrates that CQ inhibits the growth of yeast by up-regulating the expression of TDH3 to influence the cell cycle. It is expected to provide new insights for the antimicrobial mechanism of CQ.
关键词	Saccharomyces cerevisiae Clioquinol Cell cycle arrest Proteomics TDH3
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60031
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yan, Chongjia,Wang, Song,Wang, Jian,et al. Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae[J]. MICROBIOLOGICAL RESEARCH,2018,214.
APA	Yan, Chongjia.,Wang, Song.,Wang, Jian.,Li, Hui.,Huang, Zhiwei.,...&Shi, Ping.(2018).Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae.MICROBIOLOGICAL RESEARCH,214.
MLA	Yan, Chongjia,et al."Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae".MICROBIOLOGICAL RESEARCH 214(2018).