Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range

NWIPB OpenIR

	Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range
	Liu, Sijie; Dou, Leina; Yao, Xiaolin; Zhang, Wentao; Zhao, Man; Yin, Xuechi; Sun, Jing; Zhang, Daohong; Wang, Jianlong
	2020
发表期刊	BIOSENSORS & BIOELECTRONICS
卷号	169
摘要	Development of sensitive, facile and rapid biosensors is important for widespread applications. Nanozymes can be ideal signal donors for constructing dual-readout lateral flow immunoassays (LFIA) because they are an excellent class of optical reporters. Herein, a magnetic prussian blue nanozyme (MPBN) mediated dual-readout on-demand multiplex lateral flow immunoassay (MLFIA) was established by employing ractopamine (RAC) and clenbuteml (CLE) as the model analytes. The MPBN was synthesized through in-suit shell-growing and introduced as a bifunctional signal tag owing to their darker original color and peroxidase-like activity. Based on the catalytic signal created by catalyzing oxidation of chmmogenic substrate 3,3 ',5,5 '-tetramethylbenzidine (TMB) and colorimetric signal generated by tag's original color, improved precision and broadened detection range were acquired by implementing a dual-readout strategy. And a two-fold increase in the detection range could fulfill different limit requirements of the same target in various regions. The obtained recoveries from 84.01% to 119.94% indicating the repeatability and reliability of the proposed method. This method provides an attractive platform for the detection of a same target with different detection limits, which possesses a considerable potential in monitoring of other targets.
关键词	Dual-readout Multiplex lateral flow immunoassay Nanozyme Clenbuterol Ractopamine
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60214
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Sijie,Dou, Leina,Yao, Xiaolin,et al. Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range[J]. BIOSENSORS & BIOELECTRONICS,2020,169.
APA	Liu, Sijie.,Dou, Leina.,Yao, Xiaolin.,Zhang, Wentao.,Zhao, Man.,...&Wang, Jianlong.(2020).Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range.BIOSENSORS & BIOELECTRONICS,169.
MLA	Liu, Sijie,et al."Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range".BIOSENSORS & BIOELECTRONICS 169(2020).