Separation of five flavonoids with similar polarity from Caragana korshinskii Kom. by preparative high speed counter-current chromatography with recycling and heart cut mode

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	Separation of five flavonoids with similar polarity from Caragana korshinskii Kom. by preparative high speed counter-current chromatography with recycling and heart cut mode
	Ma, Yumei; Yang, Xue; Chen, Jilin; Zhao, Jingyang; Yang, Li; Yan, Shuping; Li, Hongmei; Shen, Cheng; Wei, Yangfei; Wang, Shuo; Chen, Tao; Chen, Zhi; Li, Yulin
	2020
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	43 期号:19
摘要	Five flavonoids with similar polarity were obtained from Caragana korshinskii Kom. by preparative high speed counter-current chromatography. Due to their similar polarity, it is difficult to select a suitable solvent system for one-step separation. Three fractions including one pure compound were only obtained with a relatively suitable solvent system of ethyl acetate: n-butanol:water (0.5% glacial acetic acid) (4:6:10, v:v:v). In order to improve the separation efficiency, recycling and heart cut modes were introduced. As a result, two flavonoids with separation factor value at 1.13 and the other two with separation factor value at 1.20 were successfully separated. Finally, five flavonoids with purity higher than 98% were obtained. Fortunately, a new compound named myricetin 3'-methyl ether 3-O-glucoside-7-O-rhamnoside was obtained. The results indicated that the recycling combined with heart-cut mode could be effective for the preparation of natural compounds with similar polarity.
关键词	Caragana korshinskiiKom. flavonoid glycosides high-speed counter-current chromatography recycling similar polarity
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60235
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Ma, Yumei,Yang, Xue,Chen, Jilin,et al. Separation of five flavonoids with similar polarity from Caragana korshinskii Kom. by preparative high speed counter-current chromatography with recycling and heart cut mode[J]. JOURNAL OF SEPARATION SCIENCE,2020,43(19).
APA	Ma, Yumei.,Yang, Xue.,Chen, Jilin.,Zhao, Jingyang.,Yang, Li.,...&Li, Yulin.(2020).Separation of five flavonoids with similar polarity from Caragana korshinskii Kom. by preparative high speed counter-current chromatography with recycling and heart cut mode.JOURNAL OF SEPARATION SCIENCE,43(19).
MLA	Ma, Yumei,et al."Separation of five flavonoids with similar polarity from Caragana korshinskii Kom. by preparative high speed counter-current chromatography with recycling and heart cut mode".JOURNAL OF SEPARATION SCIENCE 43.19(2020).