Integrative analysis of transcriptomic and proteomic changes related to male sterility inTagetes erecta

NWIPB OpenIR

	Integrative analysis of transcriptomic and proteomic changes related to male sterility inTagetes erecta
	Tang, Nan; Liu, Wei; Zhang, Wuhua; Tang, Daocheng
	2020
发表期刊	PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
卷号	26 期号:10
摘要	Male sterile and male fertile two-type lines are important in heterosis utilization and breeding inTagetes erecta, but the genes and pathways involved in male sterility are poorly understood. To explore these topics, transcriptome data (by RNA-seq) and proteome data (by iTRAQ) were gathered from flower buds of the male sterile line 'MS2-2' and male fertile line 'MF2-2' and integrated for a better understanding of the underlying molecular mechanisms of male sterility inT. erecta. The RNA-seq procedure generated 285,139,740 clean reads and 63359 unigenes and 6640 differentially expressed genes (DEGs) were identified, of which 4136 were downregulated and 2504 were upregulated in 'MS2-2'. DEGs related to flower development, pollen development, pollen wall assembly, endogenous hormones and transcription factors were identified. The iTRAQ analysis identified 3950 proteins in total; 789 were differentially expressed proteins (381 upregulated, 408 downregulated), which were mainly annotated to the Ribosome, Carbon metabolism and Biosynthesis of amino acids pathways. An association analysis revealed strong correlation (r Pearson = 0.6019) between the transcriptomic and proteomic data, and 256 and 34 proteins showed the same and opposite expression patterns with regard to their transcripts, respectively. Pathways such as photosynthesis, fatty acid biosynthesis and phenylpropanoid biosynthesis which influence tapetum and pollen development in male sterile plants, were significantly enriched at the transcript and protein levels. Most genes involved in these pathways were downregulated in 'MS2-2'. The low expression of these genes or functional loss of proteins could be associated with flower development, pollen development and related to changes in fertility inT. erecta. This study provided transcriptomic and proteomic information forT. erectathat could illuminate the mechanism of male sterility.
关键词	Marigold Transcriptome sequencing Proteome Male sterility
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60242
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tang, Nan,Liu, Wei,Zhang, Wuhua,et al. Integrative analysis of transcriptomic and proteomic changes related to male sterility inTagetes erecta[J]. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS,2020,26(10).
APA	Tang, Nan,Liu, Wei,Zhang, Wuhua,&Tang, Daocheng.(2020).Integrative analysis of transcriptomic and proteomic changes related to male sterility inTagetes erecta.PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS,26(10).
MLA	Tang, Nan,et al."Integrative analysis of transcriptomic and proteomic changes related to male sterility inTagetes erecta".PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS 26.10(2020).