13-Plex UHPLC-MS/MS Analysis of Hexanal and Heptanal Using Multiplex Tags Chemical Isotope Labeling Technology

NWIPB OpenIR

	13-Plex UHPLC-MS/MS Analysis of Hexanal and Heptanal Using Multiplex Tags Chemical Isotope Labeling Technology
	Hu, Jingwen; Chen, Shi-En; Zhu, Shuyun; Jia, Wenhui; Sun, Jing; Zhao, Xian-En; Liu, Huwei
	2020
发表期刊	JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷号	31 期号:9
摘要	In this work, a new series of chemical isotope labeling reagents, levofloxacin-hydrazide-based mass tags (LHMTs) named as LHMT359/360/361/362/363/364/365/366/373/375/376/378/379/381 were first designed and synthesized for the high-throughput analysis of potential biomarkers containing hexanal and heptanal of lung cancer. We exploited a new core structure of levofloxacin-d(3), which significantly enhanced the multiplexing capability. Among them, LHMT359 was used for labeling standard compounds as internal standards for quantification. Using LHMT373-heptanal as dummy template, dummy magnetic molecularly imprinted polymers (DMMIPs) were prepared for magnetic dispersive solid-phase extraction after derivatization procedure. Other 12 LHMTs were established for high-throughput labeling hexanal and heptanal in human serum samples. The presynthesized DMMIPs can selectively extract LHMTs-derivatives of hexanal and heptanal from equally mixed derivatization solutions. The enriched derivatives of hexanal and heptanal were quantified by ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). A single UHPLC-MS/MS run enabled simultaneously quantifying hexanal and heptanal from 12 serum samples only within 2 min. The limits of detection were all 0.5 pM for hexanal and heptanal. The accuracies from human serum samples ranged from -10.2% to +11.0% with the intra- and interday precisions less than 11.3%. Meanwhile, this method was successfully applied for the analysis of hexanal and heptanal in serum samples from healthy people and lung cancer patients. The results show that this method has the significant advantages of high sensitivity, accuracy, selectivity, and analysis-throughput. The method application indicates that the developed method is promising in the screening of suspected lung cancer patients.
关键词	aldehydes chemical isotope labeling multiplex mass tags magnetic molecularly imprinted polymers liquid chromatography tandem mass spectrometry lung cancer biomarkers
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60255
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Jingwen,Chen, Shi-En,Zhu, Shuyun,et al. 13-Plex UHPLC-MS/MS Analysis of Hexanal and Heptanal Using Multiplex Tags Chemical Isotope Labeling Technology[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2020,31(9).
APA	Hu, Jingwen.,Chen, Shi-En.,Zhu, Shuyun.,Jia, Wenhui.,Sun, Jing.,...&Liu, Huwei.(2020).13-Plex UHPLC-MS/MS Analysis of Hexanal and Heptanal Using Multiplex Tags Chemical Isotope Labeling Technology.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,31(9).
MLA	Hu, Jingwen,et al."13-Plex UHPLC-MS/MS Analysis of Hexanal and Heptanal Using Multiplex Tags Chemical Isotope Labeling Technology".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 31.9(2020).