Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice

NWIPB OpenIR

	Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice
	Jie, Duo; Shan, Zhongshu; Ma, Zhiliang; Zhang, Ming; Gao, Tingting; Wu, Hao; Shan, Bin; Wei, Lixin; Bi, Hongtao
	2020
发表期刊	ANNALS OF PALLIATIVE MEDICINE
卷号	9 期号:5
摘要	Background: Ma-Nuo-Xi decoction (MNXD), as well as its hundreds of derivative preparations, has been used in Tibetan medicine since the 14th century. MNXD is in accordance with the theory of treatment determination based on syndrome differentiation. This study aimed to compare the effect of the auxiliary MNXD prescription (MNXD-AD) with that of the basic MNXD prescription (MNXD-BD) on the immunostimulating activity of MNXD. Methods: The immunopotentiation of MNXD, MNXD-BD, and MNXD-AD was evaluated using a cyclophosphamide (CTX)-immunosuppressed mouse model. Their influences on non-specific and specific immunity were evaluated using immune organ indexes, peripheral white blood cell (WBC) count, red blood cell (RBC) count, platelet count, phagocytosis, macrophage-secreted nitric oxide (NO) and cytokines, natural killer (NK) cytotoxic activity, lymphocyte proliferation, serum cytokines, splenic T-lymphocyte subpopulations, and quantitative hemolysis of sheep red blood cell (QHS SRBC) assays. Results: MNXD, MNXD-BD, and MNXD-AD increased the spleen and thymus indexes, as well as the peripheral WBC, RBC, and platelet counts. They also promoted phagocytosis, NO and cytokine secretion from macrophages, NK cytotoxic activity, and lymphocyte proliferation, and also raised the CD4(+)/CD8(+) T-cell ratio, serum cytokine concentrations, and haemolysin formation in CTX-treated immunosuppressed mice. Compared with MNXD-BD and MNXD-AD, MNXD was superior in restoring the phagocytic index, concanavalin A (ConA)-induced T-lymphocyte proliferation, NO secretion from macrophages, and haemolysin formation, as well as the levels of interleukin 1 beta (IL-1 beta), and serum interleukin-2 (IL-2) and interferon gamma (INF-beta). Conclusions: MNXD, MNXD-BD, and MNXD-AD have excellent immunostimulating and myelosuppression-restoring activities on CTX-immunosuppressed mice. Among them, MNXD-AD might be an immunomodulator, which may happen to be in line with the clinical experience of Tibetan medicine physicians of using it to promote the efficacy of MNXD-BD.
关键词	Ma-Nuo-Xi decoction (MNXD) immunostimulating effect cyclophosphamide-induced immunosuppression (CTX-induced immunosuppression) Tibetan medicine
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60260
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jie, Duo,Shan, Zhongshu,Ma, Zhiliang,et al. Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice[J]. ANNALS OF PALLIATIVE MEDICINE,2020,9(5).
APA	Jie, Duo.,Shan, Zhongshu.,Ma, Zhiliang.,Zhang, Ming.,Gao, Tingting.,...&Bi, Hongtao.(2020).Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice.ANNALS OF PALLIATIVE MEDICINE,9(5).
MLA	Jie, Duo,et al."Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice".ANNALS OF PALLIATIVE MEDICINE 9.5(2020).