Efficient Separation of Five Flavonoids from Oxytropis falcata Bunge by High-Speed Counter-Current Chromatography and their Anticancer Activity

NWIPB OpenIR

	Efficient Separation of Five Flavonoids from Oxytropis falcata Bunge by High-Speed Counter-Current Chromatography and their Anticancer Activity
	Chen, Chen; Liu, Fengqin; Zhao, Jingyang; Chen, Tao; Li, Yulin; Zhang, Dejun
	2020
发表期刊	ACTA CHROMATOGRAPHICA
卷号	32 期号:3
摘要	Oxytropis falcata Bunge, known as the "king of herbs" in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation, and anthrax. However, it is difficult to efficiently isolate compounds with high purity from O. falcata because of the complexity of traditional Tibetan medicines. In this study, the 80% ethanol elution fraction from extract by AB-8 macroporous resin column chromatography was demonstrated to have anticancer activity on human hepatoma SMMC-7721 cells in vitro. Then, a high-speed counter-current chromatography (HSCCC) method was successfully established for separation of compounds by using hexano-ethyl acetate-metha- nol-water (10:4:10:10, v/v/v/v) as the solvent system. Five flavonoids (7-hydroxyflavonone [1],5,7-dihydroxy-4'methoxy flavonol [2], 5,7-dihydroxyflavanone [3], 2',4'-dihydroxychalcone [4], and 2',4'-dihydroxydihydrochalcone [5]) were obtained in one-step separation with purities of 97.7%, 98.1%, 98.3%, 99.0%, and 98.3%, respectively. Finally, anticancer activities against the growth of SMMC-7721 cells of 5 flavonoids were confirmed. The IC50 values of the separated compounds were 213.45 mu g/mL, 197.74 mu g/mL, 375.16 mu g/mL, 17.44 mu g/mL, and 136.83 mu g/mL in 24 h, respectively. The present study provided a basis for further development and utilization of this medicinal herb as a source of a new potential anticancer agent.
关键词	Oxytropis falcata Bunge high-speed counter-current chromatography flavonoids
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60269
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Chen,Liu, Fengqin,Zhao, Jingyang,et al. Efficient Separation of Five Flavonoids from Oxytropis falcata Bunge by High-Speed Counter-Current Chromatography and their Anticancer Activity[J]. ACTA CHROMATOGRAPHICA,2020,32(3).
APA	Chen, Chen,Liu, Fengqin,Zhao, Jingyang,Chen, Tao,Li, Yulin,&Zhang, Dejun.(2020).Efficient Separation of Five Flavonoids from Oxytropis falcata Bunge by High-Speed Counter-Current Chromatography and their Anticancer Activity.ACTA CHROMATOGRAPHICA,32(3).
MLA	Chen, Chen,et al."Efficient Separation of Five Flavonoids from Oxytropis falcata Bunge by High-Speed Counter-Current Chromatography and their Anticancer Activity".ACTA CHROMATOGRAPHICA 32.3(2020).