Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Derivatization-based magnetic dummy molecularly imprinted polymers integrated with 4-plex stable isotope labeling derivatization strategy for specific and rapid determination of L-hydroxyproline in human serum | |
Zhu, Shuyun; Zheng, Longfang; Sun, Luping; Jia, Wenhui; Sun, Jing; Zhao, Xian-En; Liu, Huwei | |
2020 | |
发表期刊 | ANALYTICA CHIMICA ACTA |
卷号 | 1127 |
摘要 | The specific determination of L-hydroxyproline (Hyp) can serve as a potential indicator for early clinical diagnosis of liver fibrosis. In this work, an integrated strategy based on 4-plex stable isotope labeling derivatization combined with dummy magnetic molecularly imprinted polymers (QSILD-DMMIPs) was developed for specific extraction and rapid determination of Hyp in human serum by ultra high performance liquid chromatography tandem mass spectrometry. A new series of QSILD reagents d(0)/d(1)/d(2)/d(3)-6-N-methyl-rhodamine 6G-N-hydroxysuccinimidyl formate (d(0)/d(1)/d(2)/d(3)-MRSF) were designed, synthesized and applied for the high-throughput labeling of Hyp in serum samples. The structural analogue derivative of Hyp with 6-N-ethyl-rhodamine 6G-N-hydroxysuccinimidyl formate (ERSF-Hyp) was synthesized and used as a novel dummy template to prepare DMMIPs. The DMMIPs were well characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), fourier transform infrared spectroscopy (FTIR), Brunner Emmet Teller (BET) measurements, thermogravimetric analysis (TGA), X-ray diffraction (XRD), zeta potential and adsorption experiments. All d(0)/d(1)/d(2)/d(3)-MRSF-Hyp derivatives were conveniently and specifically adsorbed by DMMIPs in magnetic dispersive solid phase extraction procedure before injection. Method validation results including linearity (0.2-100 ng mL(-1)), limits of detection and quantitation (0.05 and 0.2 ng mL(-1)), accuracy, precision, stability, matrix effect and derivatization efficiency were satisfactory. The analytical performances benefited from efficient integration of QSILD and specific DMMIPs extraction. The proposed strategy was successfully applied for Hyp determination in human serum of liver fibrosis patients and healthy controls, which was of great significance to early diagnosis. (C) 2020 Elsevier B.V. All rights reserved. |
关键词 | Hydroxyproline Liver fibrosis biomarker Multiplexed labeling derivatization Surface imprinting technique Magnetic separation Triple quadrupole mass spectrometry |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60272 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Zhu, Shuyun,Zheng, Longfang,Sun, Luping,et al. Derivatization-based magnetic dummy molecularly imprinted polymers integrated with 4-plex stable isotope labeling derivatization strategy for specific and rapid determination of L-hydroxyproline in human serum[J]. ANALYTICA CHIMICA ACTA,2020,1127. |
APA | Zhu, Shuyun.,Zheng, Longfang.,Sun, Luping.,Jia, Wenhui.,Sun, Jing.,...&Liu, Huwei.(2020).Derivatization-based magnetic dummy molecularly imprinted polymers integrated with 4-plex stable isotope labeling derivatization strategy for specific and rapid determination of L-hydroxyproline in human serum.ANALYTICA CHIMICA ACTA,1127. |
MLA | Zhu, Shuyun,et al."Derivatization-based magnetic dummy molecularly imprinted polymers integrated with 4-plex stable isotope labeling derivatization strategy for specific and rapid determination of L-hydroxyproline in human serum".ANALYTICA CHIMICA ACTA 1127(2020). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论