NWIPB OpenIR
Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening
Fang, Yi; Zhao, Chengzhen; Xiang, Hai; Jia, GongXue; Zhong, Rongzhen
2020
发表期刊REPRODUCTION IN DOMESTIC ANIMALS
卷号55期号:9
摘要Cryopreservation damages permeability of sperm mitochondrial membranes, with formation of a mitochondrial permeability transition pore (mPTP). Mitochondria are both a primary synthesis site and principle target for melatonin, which can directly inhibit mPTP formation. The objective was to determine effects of melatonin on mPTP opening of frozen-thawed ram sperm and elucidate underlying pathways by antagonist and agonists of melatonin receptors (MTs), and antagonists of PI3K and GSK 3 beta treatments; furthermore, plasma membrane integrity, mitochondrial membrane potential (Delta psi m), mitochondrial cytochrome c (Cyt c) release and fertilization were analysed to assess the effect of mPTP status mediated by melatonin on quality of frozen-thawed sperm. Fresh ram semen was diluted in glucose-egg yolk buffer with 0 or 10(-7) M melatonin (frozen and frozen + melatonin groups, respectively) and slow-frozen. In frozen-thawed sperm, melatonin added at initiation of 4 degrees C equilibration was most effective for inhibiting mPTP opening, decreasing peptidyl-prolyl-cis/trans isomerase activity of cyclophilin D and increasing plasma membrane integrity, Delta psi m, mitochondrial Cyt c concentration and fertilizing ability (p .05). In a mechanistic study, the melatonin receptor (MT)1 antagonist eliminated inhibition of melatonin on mPTP opening, whereas MT1 agonist had opposite effects (p < .05). Neither MT2 antagonist nor agonist had significant effect, but PI3K and/or GSK 3 beta antagonist decreased inhibition of MT1 agonist on mPTP opening (p .05). In conclusion, melatonin improved sperm cryopreservation, perhaps by acting on MT1 via the PI3K-Akt-GSK 3 beta pathway to inhibit mPTP opening.
关键词cryopreservation melatonin mitochondrial permeability transition pore ram sperm
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/60280
专题中国科学院西北高原生物研究所
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GB/T 7714
Fang, Yi,Zhao, Chengzhen,Xiang, Hai,et al. Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening[J]. REPRODUCTION IN DOMESTIC ANIMALS,2020,55(9).
APA Fang, Yi,Zhao, Chengzhen,Xiang, Hai,Jia, GongXue,&Zhong, Rongzhen.(2020).Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening.REPRODUCTION IN DOMESTIC ANIMALS,55(9).
MLA Fang, Yi,et al."Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening".REPRODUCTION IN DOMESTIC ANIMALS 55.9(2020).
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