Preparative isolation of arylbutanoid-type phenol [(-)-rhododendrin] with peak tailing on conventional C18 column using middle chromatogram isolated gel column coupled with reversed-phase liquid chromatography

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	Preparative isolation of arylbutanoid-type phenol [(-)-rhododendrin] with peak tailing on conventional C18 column using middle chromatogram isolated gel column coupled with reversed-phase liquid chromatography
	Dang, Jun; Du, Yurong; Wang, Qi; Dawa, YangZom; Chen, Chengbiao; Wang, Qilan; Ma, Jianbin; Tao, Yanduo
	2020
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	43 期号:16
摘要	Reversed-phase liquid chromatography coupled with middle chromatogram isolated gel column was employed for the efficient preparative separation of the arylbutanoid-type phenol [(-)-rhododendrin] fromSaxifraga tangutica. Universal C18 (XTerra C18) and XCharge C18 columns were compared for (-)-rhododendrin fraction analysis and preparation. Although tailing and overloading occurred on the XTerra C18 column, the positively charged reversed-phase C18 column (XCharge C18) overcame these drawbacks, allowing for favorable separation resolution, even when loading at a on a preparative scale (3.69 mg per injection). The general separation process was as follows. First, 365.0 mg of crude (-)-rhododendrin was enriched from 165 gSaxifraga tanguticaextract via a middle chromatogram isolated gel column. Second, separation was performed on an XTerra C18 preparative column, from which 73.8 mg of the target fraction was easily obtained. Finally, the 24.0 mg tailing peak of (-)-rhododendrin on XTerra C18 column was selectively purified on the XCharge C18 analytical column. These results demonstrate that the tailing nonalkaloid peaks can be effectively used for preparative isolation on XCharge C18 columns.
关键词	(-)-rhododendrin reversed-phase liquid chromatography Saxifraga tangutica tailing peaks
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60309
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Dang, Jun,Du, Yurong,Wang, Qi,et al. Preparative isolation of arylbutanoid-type phenol [(-)-rhododendrin] with peak tailing on conventional C18 column using middle chromatogram isolated gel column coupled with reversed-phase liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2020,43(16).
APA	Dang, Jun.,Du, Yurong.,Wang, Qi.,Dawa, YangZom.,Chen, Chengbiao.,...&Tao, Yanduo.(2020).Preparative isolation of arylbutanoid-type phenol [(-)-rhododendrin] with peak tailing on conventional C18 column using middle chromatogram isolated gel column coupled with reversed-phase liquid chromatography.JOURNAL OF SEPARATION SCIENCE,43(16).
MLA	Dang, Jun,et al."Preparative isolation of arylbutanoid-type phenol [(-)-rhododendrin] with peak tailing on conventional C18 column using middle chromatogram isolated gel column coupled with reversed-phase liquid chromatography".JOURNAL OF SEPARATION SCIENCE 43.16(2020).