Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation

NWIPB OpenIR

	Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation
	Hu, Jingwen; Zhu, Shuyun; Chen, Shi-En; Liu, Ruisheng; Sun, Jing; Zhao, Xian-En; Liu, Huwei
	2020
发表期刊	MICROCHIMICA ACTA
卷号	187 期号:7
摘要	A new series of 9-plex chemical isotope-labeling reagents, levofloxacin-based mass tags (LMTs) named as LMT359, 360, 361, 362, 363, 373, 375, 376, and 378, was firstly designed and synthesized for the high-throughput labeling of globotriaosylsphingosine (lyso-Gb3), a disease biomarker of Fabry disease. Creatively based on derivatization strategy-dummy template technique, dummy magnetic molecularly imprinted polymers (DMMIPs) were designed and prepared using LMT387-labeled lyso-Gb3 as a dummy template. The novel DMMIP material was used as sorbents for magnetic dispersive solid-phase extraction of 9-plexed LMT derivatives of lyso-Gb3 from equally mixed derivatization solutions. The enriched 8-plexed lyso-Gb3 derivatives from 8 real samples were quantified by ultra-high-performance liquid chromatography tandem mass spectrometry in a single run using simultaneously extracted LMT359-labeled standard lyso-Gb3 as internal standards. DMMIPs were characterized by using the transmission electron microscope (TEM), Fourier transform infrared, X-ray photoelectron spectroscopy, and some other characterization techniques. TEM micrograph showed that the prepared DMMIPs had an apparent imprinting layer. Triple-recognition abilities of DMMIPs towards LMT-lyso-Gb3 mainly rely on the hydrogen bonding, electrostatic attraction, hydrophobic interaction, and boronate affinity. The imprinting factor of DMMIPs towards LMT-lyso-Gb3 was 5.1. This method shows the advantages of high selectivity (triple recognition), high sensitivity, high accuracy (recovery 93.5-108.8%), and high throughput (8 samples in a single run). The proposed method was successfully applied to the determination of lyso-Gb3 in plasma samples with spiked recoveries in the range of 95.0-102.4%. This indicates that the method is promising in bioanalysis and medical testing of lyso-Gb3 in the future.Synthesis of multiplexed derivatization reagents and its correlative molecularly imprinted polymers for magnetic extraction of globotriaosylsphingosine
关键词	Globotriaosylsphingosine Fabry disease biomarker Multiplexed labeling Dummy molecular imprinting technology Magnetic material Triple quadrupole mass spectrometry
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60315
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Jingwen,Zhu, Shuyun,Chen, Shi-En,et al. Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation[J]. MICROCHIMICA ACTA,2020,187(7).
APA	Hu, Jingwen.,Zhu, Shuyun.,Chen, Shi-En.,Liu, Ruisheng.,Sun, Jing.,...&Liu, Huwei.(2020).Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation.MICROCHIMICA ACTA,187(7).
MLA	Hu, Jingwen,et al."Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation".MICROCHIMICA ACTA 187.7(2020).