Comparative study of gut microbiota in Tibetan wild asses (Equus kiang) and domestic donkeys (Equus asinus) on the Qinghai-Tibet plateau

NWIPB OpenIR

	Comparative study of gut microbiota in Tibetan wild asses (Equus kiang) and domestic donkeys (Equus asinus) on the Qinghai-Tibet plateau
	Liu, Hongjin; Zhao, Xinquan; Han, Xueping; Xu, Shixiao; Zhao, Liang; Hu, Linyong; Xu, Tianwei; Zhao, Na; Zhang, Xiaoling; Chen, Dongdong; He, Fuquan; Chen, Xin
	2020
发表期刊	PEERJ
卷号	8
摘要	Tibetan wild asses (Equus Kiang) are the only wild species of perissodactyls on the Qinghai-Tibet Plateau and appears on the International Union for Conversation of Nature (IUCN) 2012 Red List of threatened species. Therefore, understanding the gut microbiota composition and function of wild asses can provide a theoretical for the situ conservation of wild animals in the future.In this study, we measured the dry matter digestion by the 4 molar hydrochloric acid (4N HCL) acid-insoluble ash method and analyzed the intestinal microbiota of wild asses and domestic donkeys by high-throughput sequencing of the 16s rDNA genes in V3-V4 regions. The results showed that the dry matter digestion in wild asses was significantly higher than in domestic donkeys (P < 0.05). No significant difference in alpha diversity was detected between these two groups. Beta diversity showed that the bacterial community structure of wild asses was acutely different from domestic donkeys. At the phylum level, the two dominant phyla Bacteroidetes and Firmicutes in wild asses were significantly higher than that in domestic donkeys. At the genus level, Ruminococcaceae_NK4A214, Phascolarctobacterium, Coprostanoligenes_group, Lachnospiraceae_XPB1014_group and Akkermansia in wild asses were significantly higher than in domestic donkeys. Moreover, statistical comparisons showed that 40 different metabolic pathways exhibited significant differences. Among them, 29 pathways had richer concentrations in wild asses than domestic donkeys, mainly included amino acid metabolism, carbohydrate metabolism, and energy metabolism. Of note, network analysis showed that wild asses harbored a relatively more complex bacterial network than domestic donkeys, possibly reflecting the specific niche adaption of gut bacterial communities through species interactions. The overall results indicated that wild asses have advantages over domestic donkeys in dry matter digestion, gut microbial community composition and function, and wild asses have their unique intestinal flora to adapt high altitudes on the Qinghai-Tibet plateau.
关键词	Qinghai-Tibet plateau Tibetan wild asses Domestic donkeys 16S ribosomal RNA gene Gut microbiota Acid-insoluble ash
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60318
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Hongjin,Zhao, Xinquan,Han, Xueping,et al. Comparative study of gut microbiota in Tibetan wild asses (Equus kiang) and domestic donkeys (Equus asinus) on the Qinghai-Tibet plateau[J]. PEERJ,2020,8.
APA	Liu, Hongjin.,Zhao, Xinquan.,Han, Xueping.,Xu, Shixiao.,Zhao, Liang.,...&Chen, Xin.(2020).Comparative study of gut microbiota in Tibetan wild asses (Equus kiang) and domestic donkeys (Equus asinus) on the Qinghai-Tibet plateau.PEERJ,8.
MLA	Liu, Hongjin,et al."Comparative study of gut microbiota in Tibetan wild asses (Equus kiang) and domestic donkeys (Equus asinus) on the Qinghai-Tibet plateau".PEERJ 8(2020).