NWIPB OpenIR
Establishment of a feeder and serum-free culture system for human embryonic stem cells
Wang, LiYun; Zhang, RuiNa; Ma, RongHua; Jia, GongXue; Jian, ShengYan; Zeng, XiangHui; Xiong, ZhengFang; Li, BinYe; Li, Chen; Lv, ZhenZhen; Bai, Xue
2020
发表期刊ZYGOTE
卷号28期号:3
摘要Stem cells are an immortal cell population capable of self-renewal; they are essential for human development and ageing and are a major focus of research in regenerative medicine. Despite considerable progress in differentiation of stem cellsin vitro, culture conditions require further optimization to maximize the potential for multicellular differentiation during expansion. The aim of this study was to develop a feeder-free, serum-free culture method for human embryonic stem cells (hESCs), to establish optimal conditions for hESC proliferation, and to determine the biological characteristics of the resulting hESCs. The H9 hESC line was cultured using a homemade serum-free, feeder-free culture system, and growth was observed. The expression of pluripotency proteins (OCT4, NANOG, SOX2, LIN28, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81) in hESCs was determined by immunofluorescence and western blotting. The mRNA expression levels of genes encoding nestin, brachyury and alpha-fetoprotein in differentiated H9 cells were determined by RT-PCR. The newly developed culture system resulted in classical hESC colonies that were round or elliptical in shape, with clear and neat boundaries. The expression of pluripotency proteins was increased, and the genes encoding nestin, brachyury, and alpha-fetoprotein were expressed in H9 cells, suggesting that the cells maintainedin vitrodifferentiation capacity. Our culture system containing a unique set of components, with animal-derived substances, maintained the self-renewal potential and pluripotency of H9 cells for eight passages. Further optimization of this system may expand the clinical application of hESCs.
关键词Basic fibroblast growth factor Feeder-free Human embryonic stem cells Pluripotency Self-renewal
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/60320
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Wang, LiYun,Zhang, RuiNa,Ma, RongHua,et al. Establishment of a feeder and serum-free culture system for human embryonic stem cells[J]. ZYGOTE,2020,28(3).
APA Wang, LiYun.,Zhang, RuiNa.,Ma, RongHua.,Jia, GongXue.,Jian, ShengYan.,...&Bai, Xue.(2020).Establishment of a feeder and serum-free culture system for human embryonic stem cells.ZYGOTE,28(3).
MLA Wang, LiYun,et al."Establishment of a feeder and serum-free culture system for human embryonic stem cells".ZYGOTE 28.3(2020).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, LiYun]的文章
[Zhang, RuiNa]的文章
[Ma, RongHua]的文章
百度学术
百度学术中相似的文章
[Wang, LiYun]的文章
[Zhang, RuiNa]的文章
[Ma, RongHua]的文章
必应学术
必应学术中相似的文章
[Wang, LiYun]的文章
[Zhang, RuiNa]的文章
[Ma, RongHua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。