Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography

NWIPB OpenIR

	Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography
	Chen, Chen; Zhao, Jingyang; Chen, Jilin; Chi, Ming; Liu, Yuchao
	2020
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	58 期号:3
摘要	Oxytropis falcata Bunge, known as "the King of Herbs" in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation and anthrax. However, it is difficult to isolate compound with high-purity from O. falcata because of its complexity. In this work, an efficient method was successfully established for the separation of 3-hydroxy-3-methylglutaryl (HMG) flavonoid glycosides from O. falcata by 2D preparative chromatography (2D-HPLC). An ODS C-18 preparative column was used for the first-dimension preparation, and the XCharge C-18 preparative column with different separation selectivity to the ODS C-18 stationary phase was used for the second-dimension preparation. Four HMG flavonoid glycosides (oxytroflavosides A-D) and two flavonoid glycosides (oxytroflavosides F and G) were separated with purities over 98%. Their structures were elucidated by nuclear magnetic resonance spectroscopy. The 2D-HPLC method used in this study is effective for preparative separation of HMG flavonoid glycosides from O. falcata. Additionally, this method shows great potential for the separation of flavonoid glycosides from other plant extracts.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60390
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Chen,Zhao, Jingyang,Chen, Jilin,et al. Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2020,58(3).
APA	Chen, Chen,Zhao, Jingyang,Chen, Jilin,Chi, Ming,&Liu, Yuchao.(2020).Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE,58(3).
MLA	Chen, Chen,et al."Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE 58.3(2020).