The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid

NWIPB OpenIR

	The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid
	Li, Yong-Chang; Wang, Guo-Wen; Xu, Shang-Rong; Zhang, Xiao-Na; Yang, Qi-En
	2020
发表期刊	THERIOGENOLOGY
卷号	144
摘要	Interspecies hybridization exists widely in nature and plays an important role in animal evolution and adaptation. It is commonly recognized that male offspring of interspecies hybrid are often sterile, which presents a crucial way of reproductive isolation. Currently, the mechanisms underlying interspecies hybrid male sterility are not well understood. Cattle-yak, progeny of yak (Bos grunniens) and cattle (Bos taurus) cross, is a unique animal model for investigating hybrid male sterility. Because histone modifications are vital for spermatogenesis, herein, we examined expressions of histone methyltransferases (HMTs) and distributions of histone methylations in the yak and cattle-yak testis. Histological examination of seminiferous tubules revealed that gonocytes and spermatocytes were established normally, however, spermatogenesis was arrested at the meiosis phase began at 10 months after birth in the hybrids. SUV420H1 was the only HMT examined showing a significant enrichment in cattle-yak testes at 3 months. Relative expressions of MLL5, SETDB1 and SUV420H1 were increased while SETDB2 and EZH2 were decreased in cattle-yak testes at 10 months. Relative concentrations of MLL5 and SUV420H1 were again increased while EHM72 and PRDM9 expressions were decreased at 24 months. Immunofluorescent detection of selected histone methylations in cross-sections of testicular tissues or meiotic chromosomes demonstrated that depletion of H3K4me3 and significant enrichment of H3K27me3 and H4K20me3 were observed in Sertoli cells of cattle-yak. Levels and localizations of H3K4me3, H3K9me1, H3K9me3 and H4K20me3 were strikingly different in meiotic chromosomes of cattle-yak spermatocytes. These results highlighted the potential roles of histone methylations in spermatogenic failure and hybrid male sterility. (C) 2020 Elsevier Inc. All rights reserved.
关键词	Yak Hybrid male sterility Histone methylation Spermatogenesis Meiosis
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60398
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Yong-Chang,Wang, Guo-Wen,Xu, Shang-Rong,et al. The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid[J]. THERIOGENOLOGY,2020,144.
APA	Li, Yong-Chang,Wang, Guo-Wen,Xu, Shang-Rong,Zhang, Xiao-Na,&Yang, Qi-En.(2020).The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid.THERIOGENOLOGY,144.
MLA	Li, Yong-Chang,et al."The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid".THERIOGENOLOGY 144(2020).