A FRET-based ratiometric fluorescent probe for sulfide detection in actual samples and imaging in Daphnia magna

NWIPB OpenIR

	A FRET-based ratiometric fluorescent probe for sulfide detection in actual samples and imaging in Daphnia magna
	Yang, Lei; Wang, Feng; Luo, Xianzhu; Kong, Xiaojian; Sun, Zhiwei; You, Jinmao
	2020
发表期刊	TALANTA
卷号	209
摘要	Sulfide plays an important role in many important life processes, and abnormal levels of sulfide that may cause diseases. Sulfide is also essential in industrial production and food processing, and it is well concerned for environmental issues and food safety. In order to study the physiological and pathological effects of sulfide and the impact on the environment, it is still urgent to develop a convenient and effective sulfide detection technology. Here, we developed a ratiometric fluorescent probe 7-Nitro-1,2,3-benzoxoxadiazole-Acridoneacetylpiperazine (NBD-AAP) which is based on the fluorescence resonance energy transfer (FRET) between acridone and NBD fluorophores. The NBD-AAP probe could produce a ratiometric response to micromolar sulfide in buffer (pH = 7.4), exhibiting a significant enhancement in fluorescent emission ratio (F-427/F-552) and obvious visual phenomenon (orange to pink under visible light and yellow to blue under UV light). At the same time, this NBD-AAP probe also has excellent properties including high selectivity and low detection limit (0.19 mu M). In addition, this probe has been successfully used for detecting the sulfide in actual samples (monosodium glutamate, beer, environmental water) and imaging in Daphnia magna. These results indicate that NBD-AAP has broad application prospects in sulfide detection and in vivo imaging studies.
关键词	Fluorescent probe Sulfide Ratiometric Fluorescence resonance energy transfer (FRET) Actual samples Daphnia magna
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60399
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Lei,Wang, Feng,Luo, Xianzhu,et al. A FRET-based ratiometric fluorescent probe for sulfide detection in actual samples and imaging in Daphnia magna[J]. TALANTA,2020,209.
APA	Yang, Lei,Wang, Feng,Luo, Xianzhu,Kong, Xiaojian,Sun, Zhiwei,&You, Jinmao.(2020).A FRET-based ratiometric fluorescent probe for sulfide detection in actual samples and imaging in Daphnia magna.TALANTA,209.
MLA	Yang, Lei,et al."A FRET-based ratiometric fluorescent probe for sulfide detection in actual samples and imaging in Daphnia magna".TALANTA 209(2020).