Molecular karyotyping of Siberian wild rye (Elymus sibiricus L.) with oligonucleotide fluorescence in situ hybridization (FISH) probes

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	Molecular karyotyping of Siberian wild rye (Elymus sibiricus L.) with oligonucleotide fluorescence in situ hybridization (FISH) probes
	Xie, Jihong; Zhao, Yan; Yu, Linqing; Liu, Ruijuan; Dou, Quanwen
	2020
发表期刊	PLOS ONE
卷号	15 期号:1
摘要	Siberian wild rye (Elymus sibiricus L.), an allotetraploid species, is a potentially high-quality perennial forage crop native to temperate regions. We used fluorescently conjugated oligonucleotides, representing ten repetitive sequences, including 6 microsatellite repeats, two satellite repeats, and two ribosomal DNAs, to characterize E. sibiricus chromosomes, using sequential fluorescence in situ hybridization and genomic in situ hybridization assays. Our results showed that microsatellite repeats (AAG)(10) or (AGG)(10), satellite repeats pAs1 and pSc119.2, and ribosomal 5S rDNA and 45S rDNA are specific markers for unique chromosomes. A referable karyotype ideogram was suggested, by further polymorphism screening, across different E. sibiricus cultivars with a probe mixture of (AAG)(10), Oligo-pAs1, and OligopSc119.2. Chromosomal polymorphisms vary between different genomes and between different individual chromosomes. In particular, two distinct forms of chromosome E in H genome were identified in intra- and inter-populations. Here, the significance of these results, for E. sibiricus genome research and breeding, and novel approaches to improve fluorescence in situ hybridization-based karyotyping are discussed.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60436
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xie, Jihong,Zhao, Yan,Yu, Linqing,et al. Molecular karyotyping of Siberian wild rye (Elymus sibiricus L.) with oligonucleotide fluorescence in situ hybridization (FISH) probes[J]. PLOS ONE,2020,15(1).
APA	Xie, Jihong,Zhao, Yan,Yu, Linqing,Liu, Ruijuan,&Dou, Quanwen.(2020).Molecular karyotyping of Siberian wild rye (Elymus sibiricus L.) with oligonucleotide fluorescence in situ hybridization (FISH) probes.PLOS ONE,15(1).
MLA	Xie, Jihong,et al."Molecular karyotyping of Siberian wild rye (Elymus sibiricus L.) with oligonucleotide fluorescence in situ hybridization (FISH) probes".PLOS ONE 15.1(2020).