Comparison of Profiling of Hairy Root of Two Tibetan Medicinal Plants Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim.

NWIPB OpenIR

	Comparison of Profiling of Hairy Root of Two Tibetan Medicinal Plants Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim.
	Lei, Tianxiang; Wang, Huan; Li, Songling; Cai, Xiaojian; Chen, Shilong; Cheng, Tingfeng; Shen, Jianwei; Shi, Shengbo; Zhou, Dangwei
	2020
发表期刊	CURRENT PHARMACEUTICAL BIOTECHNOLOGY
卷号	21 期号:6
摘要	Background: Tropane Alkaloids (TAs) are important drugs for curing many diseases in the medical industry. Methods: To sustainably exploit TA resources in endangered traditional Tibetan herbs, the hairy root (HR) systems of Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim. were compared under the same culture conditions. Results: The results indicated that both the Agrobacterium rhizogenes strains and explants affected the HR induction frequency. MSU440, A4 and LBA9402 strains could induce hairy roots following infection of cotyledon and hypocotyl of A. tanguticus while LBA9402 could not induce HR on either explants of P. tangutica. The efficiency of LBA9402 was higher than A4 and MSU440 on A. tanguticus and A4 was better strain than MSU440 on P. tangutica. The hypocotyl explant was more suitable for P. tangutica and cotyledon explant was better for A. tangutica with a transformation frequency of 33.3% (P. tangutica) and 82.5% (A. tanguikus), respectively. In a flask reactor system, both the growth curves of HR for two species both appeared to be "S" curve; however, the HR of P. tangutica grew more rapidly than that of A. tanguticus, and the latter accumulated more biomass than the former. As the culture volume increased, the HR proliferation coefficient of both the species increased. HPLC analysis results showed that the content of TAs in the HR of P. tangutica was 257.24mg/1008.DW, which was more than that of A. tanguticus HR (251.08mg/100g.DW), and the anisodamine in the Pt-HR was significantly higher than that in At-HR. Moreover, tropane alkaloids in the HR of the two species were all significantly higher than that of the roots of aseptic seedlings. Conclusion: Our results suggest that HR of P. tangutica and A. tanguticus both could provide a useful platform for sustainable utilization of two Tibetan medicinal plants in the Qinghai-Tibetan Plateau in the future.
关键词	Przewalskia tangutica Maxim. Anisodus tanguticus Maxim. Agrobacterium rhizogenes growth curve hairy roots tropane alkaloids
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60456
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Lei, Tianxiang,Wang, Huan,Li, Songling,et al. Comparison of Profiling of Hairy Root of Two Tibetan Medicinal Plants Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim.[J]. CURRENT PHARMACEUTICAL BIOTECHNOLOGY,2020,21(6).
APA	Lei, Tianxiang.,Wang, Huan.,Li, Songling.,Cai, Xiaojian.,Chen, Shilong.,...&Zhou, Dangwei.(2020).Comparison of Profiling of Hairy Root of Two Tibetan Medicinal Plants Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim..CURRENT PHARMACEUTICAL BIOTECHNOLOGY,21(6).
MLA	Lei, Tianxiang,et al."Comparison of Profiling of Hairy Root of Two Tibetan Medicinal Plants Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim.".CURRENT PHARMACEUTICAL BIOTECHNOLOGY 21.6(2020).