A peptide-based fluorescent sensor for selective imaging of glutathione in living cells and zebrafish

NWIPB OpenIR

	A peptide-based fluorescent sensor for selective imaging of glutathione in living cells and zebrafish
	Li, Yongxin; Di, Cuixia; Wu, Jiang; Si, Jing; Chen, Yuhong; Zhang, Hong; Ge, Yushu; Liu, Dan; Liu, Weisheng
	2020
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷号	412 期号:2
摘要	Monitoring and imaging glutathione (GSH) in living systems is an essential tool to determine the key roles of GSH in biological pathways, but most fluorescent sensors can only be used in vitro because of their potential biotoxicity. Here, a peptide-based fluorescent sensor, FP, has been successfully designed and synthesized based on the biocompatibility of the peptide backbone and low toxicity. The design strategy of FP contains a specific spatial structure of the peptide sequence which selectively binds to Cu2+, triggering fluorescence quenching. Interestingly, the fluorescence of FP can be fully restored by GSH, due to the strong binding between Cu2+ and the GSH sulfhydryl groups. Finally, the sensor is highly sensitive and selective for imaging GSH both in vitro and in vivo with low toxicity. Thus, FP with its strong "on-off-on" fluorescence changes is a powerful way to image GSH both in cells and zebrafish larvae to study the GSH pathway.
关键词	Peptide sensor On-off-on Cu2+ Glutathione Bioimaging
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60464
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Yongxin,Di, Cuixia,Wu, Jiang,et al. A peptide-based fluorescent sensor for selective imaging of glutathione in living cells and zebrafish[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020,412(2).
APA	Li, Yongxin.,Di, Cuixia.,Wu, Jiang.,Si, Jing.,Chen, Yuhong.,...&Liu, Weisheng.(2020).A peptide-based fluorescent sensor for selective imaging of glutathione in living cells and zebrafish.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,412(2).
MLA	Li, Yongxin,et al."A peptide-based fluorescent sensor for selective imaging of glutathione in living cells and zebrafish".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 412.2(2020).