Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stresses

NWIPB OpenIR

	Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stresses
	Zhang, Y-M; Wang, H-Q; Liu, D-M; Liu, R-J
	2020
发表期刊	BIOLOGIA PLANTARUM
卷号	64
摘要	the LEA protein superfamily, group 4 members arc characterized by a conserved N -terminal region and a stnicturally disordered C -terminal region that varies regarding length and amino acid content. Previous in vitro assays have suggested that the conserved N -terminal region shared by group 4 LEA proteins is critical for forming an amphipathic a-helix and protecting enzymatic activities from the adverse effects of desiccation or freezing. However, the cellular roles of the varying C-tenninal region remain largely to be characterized. Medicago truncatula contains five subgroup LEA4B proteins encoding loci of which three are tandemly arranged on chromosome 7 due to local gene duplication events. In this study, abiotic stresses and addition of abscisic acid (ABA) induced the transcription of the four LE44B genes. Escherichia cob cells overexpressing the three tandemly aligned LEA genes indicated significantly increased tolerance to salt, osmotic, heat, and freezing stresses. However, the extent of the protective effects on the survival and growth of bacterial cells differed among the LEA proteins, potentially because of variations in the C -terminal region. This possibility was further supported by the observation that the protective effects of the native tnincated MtLEA3140, which only contains a conserved N -terminal region, were inferior to the effects of the full-length mutant version. The results suggest that the stnicturally flexible C -terminal region of group 4 LEA proteins plays roles in protecting cells from damages caused by various abiotic stresses, and provide clues for elucidating the mechanisms underlying the intracellular functions of these proteins.
关键词	abscisic acid barrel medic cold C -terminal region Escherichia colt heat late embryogenesis abundant protein salinity
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60467
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Y-M,Wang, H-Q,Liu, D-M,et al. Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stresses[J]. BIOLOGIA PLANTARUM,2020,64.
APA	Zhang, Y-M,Wang, H-Q,Liu, D-M,&Liu, R-J.(2020).Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stresses.BIOLOGIA PLANTARUM,64.
MLA	Zhang, Y-M,et al."Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stresses".BIOLOGIA PLANTARUM 64(2020).