Proteomic changes in response to lipin1 overexpression in 293T human renal epithelial cells

NWIPB OpenIR

	Proteomic changes in response to lipin1 overexpression in 293T human renal epithelial cells
	Wang, Jian; Lv, Xiaoguang; Sun, Jing; Peng, Min; Shi, Ping
	2019
发表期刊	INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
卷号	56 期号:6
摘要	Lipin1, a member of the lipin family, serves as a phospholipid phosphatase or a co-transcriptional regulator in lipid metabolism. Recent studies also show that lipin1 is involved in many other cellular metabolism processes. However, the clear regulatory mechanism for lipin1 is unknown. The 293T human renal epithelial cell line represents a commonly used and well established expression system for recombinant proteins. Herein, we used two-dimensional polyacrylamide gel electrophoresis (2D-GE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to explore the changes in protein expression induced by lipin1 overexpression in 293T cells. Western blotting was used to confirm one of the expression changes of related proteins. Subsequently, the function and relationship of these proteins were analyzed by bioinformatics approach. By using 2D-PAGE, approximately 152 proteins were separated and eleven proteins were found to be significantly affected by lipin1 overexpression compared to the control. Among them, three proteins (eEF-1B gamma, CCT1 and CCT3) were up-regulated and other eight proteins (NDKA, Stathmin, HNRNP A1, TK, KRT1, PKM, RanBP1 and LDHB) were down-regulated. These proteins were successfully identified with peptide mass fingerprinting using MALDI-TOF-MS after in-gel trypsin digestion. The bioinformatic analysis showed that these proteins are classified into seven protein species, including transferase, cleavage enzyme, cytoskeleton protein, chaperone protein, regulatory protein, structural protein and oxidoreductase. The results highlight the potential roles of lipin1 involved in many cellular metabolism processes, including myelin synthesis, extracellular domain formation, membrane bound vesicle synthesis and companion protein T complex synthesis.
关键词	293T cells Lipin1 MALDI-TOF-MS Overexpression Proteomics RanBP1
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60481
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Jian,Lv, Xiaoguang,Sun, Jing,et al. Proteomic changes in response to lipin1 overexpression in 293T human renal epithelial cells[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2019,56(6).
APA	Wang, Jian,Lv, Xiaoguang,Sun, Jing,Peng, Min,&Shi, Ping.(2019).Proteomic changes in response to lipin1 overexpression in 293T human renal epithelial cells.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,56(6).
MLA	Wang, Jian,et al."Proteomic changes in response to lipin1 overexpression in 293T human renal epithelial cells".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 56.6(2019).