The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example

NWIPB OpenIR

	The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example
	Zou, Denglang; Cui, Yunbin; Li, Si; Sang, Duocheng; Liu, Weimeng; Zhao, Tianshu; Gu, Xueli; Chen, Tao; Li, Yulin
	2021
发表期刊	JOURNAL OF SEPARATION SCIENCE
摘要	Biosynthesis is a promising way to manufacture desired products, however, the purification of its final products is a tough work due to the huge amount of reaction matrix. Liquid stationary phase of high-speed counter-current chromatography could easily avoid the commonly disadvantages that occurred in traditional column chromatography in the field of biosynthesized products purification. This characteristic makes high-speed counter-current chromatography particularly applicable for final products separation in biosynthesis. In this study, the glycosylation products of Silybin B by one-pot glycosylation were successfully purified by high-speed counter-current chromatography to show the applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products. An optimized n-hexane/ethyl acetate/methanol/water (2:5:2:3, v/v/v/v) system was applied in this study. As a result, four Silybin B glycosylation products, including 7 mg of Silybin B-5-O-beta-D-glucoside (SG-1), 12 mg of Silybin B-3-O-beta-D-glucoside (SG-2), 10 mg of Silybin B-7-O-beta-D-glucoside (SG-3), and 24 mg of Silybin B-20-O-beta-D-glucoside (SG-4), were simultaneously separated from 200 mg of glycosylation crude products, with the purity of 89.3, 95.2, 96.4, and 97.5%, respectively. Their structures were identified by spectroscopic analysis.
关键词	biosynthesis glycosylation high-speed counter-current chromatography Silybin B
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60677
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zou, Denglang,Cui, Yunbin,Li, Si,et al. The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example[J]. JOURNAL OF SEPARATION SCIENCE,2021.
APA	Zou, Denglang.,Cui, Yunbin.,Li, Si.,Sang, Duocheng.,Liu, Weimeng.,...&Li, Yulin.(2021).The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example.JOURNAL OF SEPARATION SCIENCE.
MLA	Zou, Denglang,et al."The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example".JOURNAL OF SEPARATION SCIENCE (2021).