Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography

NWIPB OpenIR

	Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography
	Chen, Tao; Liu, Yongling; Wang, Shuo; Wei, Yangfei; Song, Zhibo; Yan, Shuping; Shen, Cheng; Li, Hongmei; Li, Peipei; Li, Yulin
	2021
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
摘要	In this study, eight phenolic compounds were obtained from the over-ground parts of the Aconitum pendulum (A. pendulum) Busch through an efficient high-speed counter-current chromatography (HSCCC?method. First, the column chromatography for macroporous resin was used for the treatment of crude extract of over-ground parts of A. pendulum Busch. And then, 30% ethanol elution fraction was transferred to HSCCC for separation using the classic ethyl acetate, n-butanol and water system with the solvent ratio of 7:3:10. To improve parting efficiency, a mode of repeated injection was developed. Two groups of compounds existing in one HPLC peak were effectively separated by HSCCC. The results revealed that HSCCC could effectively isolate compounds with the same or similar polarity. Preparative HPLC was used to perform further purification, and then eight phenolic compounds were separated from the over-ground parts of the A. pendulum Busch for the first time. The current investigation successfully provides a reference for phytochemical research and, comprehensive development and utilization of A. pendulum Busch in the future. Furthermore, HSCCC efficiently separated compounds with similar polarity and its combination with preparative HPLC could play a great role in the separation of natural materials.
关键词	Aconitum pendulum busch first separation counter-current chromatography repeated injection phenolic compounds
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60687
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Tao,Liu, Yongling,Wang, Shuo,et al. Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2021.
APA	Chen, Tao.,Liu, Yongling.,Wang, Shuo.,Wei, Yangfei.,Song, Zhibo.,...&Li, Yulin.(2021).Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	Chen, Tao,et al."Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2021).