Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium-pressure liquid chromatography and reversed-phase liquid chromatography

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	Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium-pressure liquid chromatography and reversed-phase liquid chromatography
	Dang, Jun; Wang, Qi; Wang, Qilan; Yuan, Chen; Li, Gang; Ji, Tengfei
	2021
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	44 期号:20
摘要	Saxifraga tangutica is widely used as a medicinal herb to treat hepatic diseases. Here, we developed a class separation method to separate gallic acid derivatives 1,1-diphenyl-2-picrylhydrazyl inhibitors from the methanol extract of Saxifraga tangutica. Firstly, an MCI GEL CHP20P medium-pressure liquid chromatography was used to pretreat the crude extract from Saxifraga tangutica (500 g) and the target sample (fraction Fr1, 1.7 g) was obtained. Then, an online reversed-phase liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay was employed for recognizing potential 1,1-diphenyl-2-picrylhydrazyl inhibitors and six 1,1-diphenyl-2-picrylhydrazyl inhibitors fractions were recognized from fraction Fr1. Subsequently, the six 1,1-diphenyl-2-picrylhydrazyl inhibitors fractions were isolated via a ReproSil-Pur C18 AQ preparative column. During the separation process, the hydrophilic liquid chromatography was used to enrich the target compounds (Fr1-3-1-1 and Fr1-3-1-2) from the fraction Fr1-3, which were hardly isolated only by one step reversed-phase liquid chromatography. Finally, six gallic acid derivatives were obtained and identified as gallic acid (Fr1-1-1), gallic acid 3-O-beta-D-glucoside (Fr1-1-2), protocatechuic acid (Fr1-2), 4-O-galloyl-(-)-shikimic acid (Fr1-3-1-1), 5-O-galloyl-(-)-shikimic acid (Fr1-3-1-2), and 3-O-galloyl-shikimic acid (Fr1-4), respectively. Thus, the present study indicated that this method was highly efficient for the preparative separation of gallic acid derivatives 1,1-diphenyl-2-picrylhydrazyl inhibitors from natural products.
关键词	1 1-diphenyl-2-picrylhydrazyl inhibitors gallic acid derivatives preparative isolation Saxifraga tangutica
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60722
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Dang, Jun,Wang, Qi,Wang, Qilan,et al. Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium-pressure liquid chromatography and reversed-phase liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2021,44(20).
APA	Dang, Jun,Wang, Qi,Wang, Qilan,Yuan, Chen,Li, Gang,&Ji, Tengfei.(2021).Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium-pressure liquid chromatography and reversed-phase liquid chromatography.JOURNAL OF SEPARATION SCIENCE,44(20).
MLA	Dang, Jun,et al."Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium-pressure liquid chromatography and reversed-phase liquid chromatography".JOURNAL OF SEPARATION SCIENCE 44.20(2021).