Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry | |
Dong, Qi; Hu, Na; Yue, Huilan; Wang, Honglun; Ku, Jinliang | |
2021 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES |
卷号 | 1181 |
摘要 | Extracts from the bran of Chenopodium quinoa Willd. (QBE) were reported to be active in inhibiting alpha-glycosidase, a promising target for treatment of diabetes mellitus. However, the constituents responsible for the alpha-glucosidase-inhibiting activity of QBE have not been fully characterized. The present study aimed to set up a method for rapid identification of glycosidase inhibiting compounds from the quinoa bran. With surface plasmon resonance (SPR) coupled with liquid chromatography-mass spectrometry (LC-MS), we identified eight flavonoids and ten triterpenoid saponins that may bind to the alpha-glycosidase. Analysis of the interaction kinetics by molecular docking supported their alpha-glucosidase-inhibiting activity and revealed the potential mechanisms for the inhibitory effects. In summary, this study established a SPR and LC-MS-based method for rapid in vitro screening of alpha-glucosidase inhibitors and suggested the quinoa bran a potential natural source of alpha-glucosidase inhibitors. |
关键词 | Chenopodium quinoa Willd alpha-glucosidase inhibitors Surface plasmon resonance Liquid chromatography-mass spectrometry Molecular docking analysis |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60725 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Dong, Qi,Hu, Na,Yue, Huilan,et al. Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2021,1181. |
APA | Dong, Qi,Hu, Na,Yue, Huilan,Wang, Honglun,&Ku, Jinliang.(2021).Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1181. |
MLA | Dong, Qi,et al."Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1181(2021). |
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