Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells

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	Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells
	Tie, Fangfang; Ding, Jin; Hu, Na; Dong, Qi; Chen, Zhi; Wang, Honglun
	2021
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:16
摘要	Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases which lacks ideal treatment options. Kaempferol and kaempferide, two natural flavonol compounds isolated from Hippophae rhamnoides L., were reported to exhibit a strong regulatory effect on lipid metabolism, for which the mechanism is largely unknown. In the present study, we investigated the effects of kaempferol and kaempferide on oleic acid (OA)-treated HepG2 cells, a widely used in vitro model of NAFLD. The results indicated an increased accumulation of lipid droplets and triacylglycerol (TG) by OA, which was attenuated by kaempferol and kaempferide (5, 10 and 20 mu M). Western blot analysis demonstrated that kaempferol and kaempferide reduced expression of lipogenesis-related proteins, including sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD-1). Expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT enhancer binding proteins beta (C/EBP beta), two adipogenic transcription factors, was also decreased by kaempferol and kaempferide treatment. In addition, western blot analysis also demonstrated that kaempferol and kaempferide reduced expression of heme oxygenase-1 (HO-1) and nuclear transcription factor-erythroid 2-related factor 2 (Nrf2). Molecular docking was performed to identify the direct molecular targets of kaempferol and kaempferide, and their binding to SCD-1, a critical regulator in lipid metabolism, was revealed. Taken together, our findings demonstrate that kaempferol and kaempferide could attenuate OA-induced lipid accumulation and oxidative stress in HepG2 cells, which might benefit the treatment of NAFLD.
关键词	NAFLD kaempferol kaempferide lipid accumulation oxidative stress molecular docking
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60754
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tie, Fangfang,Ding, Jin,Hu, Na,et al. Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(16).
APA	Tie, Fangfang,Ding, Jin,Hu, Na,Dong, Qi,Chen, Zhi,&Wang, Honglun.(2021).Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(16).
MLA	Tie, Fangfang,et al."Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.16(2021).