Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography

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	Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography
	Dang, Jun; Ma, Jianbin; Du, Yurong; Dawa, YangZom; Wang, Qi; Chen, Chengbiao; Wang, Qilan; Tao, Yanduo; Ji, Tengfei
	2021
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷号	1170
摘要	In this study, polyamide and MCI GEL? CHP20P were employed as stationary phases in medium pressure chromatography (MPC) for the efficient preparative separation of bergenin from Saxifraga atrata. Ethanol?water, methanol-water, and acetonitrile-water mobile phases all showed good enrichment capacity for bergenin fraction when polyamide was used as a stationary phase. After 5 cycles of polyamide MPC using acetonitrile/water, 1.2 g of bergenin fraction was isolated from 180 g Saxifraga atrata herb. Further purification of this fraction was conducted using MCI GEL? CHP20P styrene-divinylbenzene beads. The bergenin fraction was separated into two fractions, and after three runs of MPC, 714.2 mg of bergenin with purity above 99% was obtained. The results demonstrate that the combination of polyamide and styrene-divinylbenzene MPC can be utilized for preparative isolation of compounds from natural products with high yield and purity.
关键词	Saxifraga atrata Polyamide Micro gel chp20p Medium pressure chromatography Bergenin
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60815
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Dang, Jun,Ma, Jianbin,Du, Yurong,et al. Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2021,1170.
APA	Dang, Jun.,Ma, Jianbin.,Du, Yurong.,Dawa, YangZom.,Wang, Qi.,...&Ji, Tengfei.(2021).Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1170.
MLA	Dang, Jun,et al."Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1170(2021).