Ce4+-triggered cascade reaction for ratiometric fluorescence detection of alendronate

NWIPB OpenIR

	Ce4+-triggered cascade reaction for ratiometric fluorescence detection of alendronate
	Xia, Meng; Shi, Fengjin; Xia, Yinghui; Sun, Jing; Zhao, Xian-En; Zhu, Shuyun
	2021
发表期刊	SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷号	251
摘要	A ratiometric fluorescence assay for alendronate (ALDS) has been designed with Ce4+-triggered cascade chromogenic reaction. This strategy involves three processes: (1) Ce4+ oxidizes ascorbic acid (AA) into dehydroascorbic acid (DHAA), which then condenses with o-phenlenediamine (OPD) to generate fluorescent 3(dihydroxyethyl)furo[3,4-b] quinoxaline-1-one (DFQ), presenting the maximum emission at 434 nm; (2) As oxidase-mimics, Ce4+ can oxidize OPD into fluorescent 2,3-diaminophenazine (DAP) which shows a strong emission at 568 nm; (3) ALDS inhibits the oxidation ability of Ce4+ towards OPD, thus inhibiting the generation of DAP. Accordingly, a homogeneous ratiometric fluorescence system with dual emission comes into being and the presence of ALDS can change the fluorescence intensity ratio obviously. With F434/F568 as readout, ALDS can be detected sensitively with the detection limit of 30 nM. Moreover, this ratiometric method was used to analyze ALDS in both human serum and pharmaceutical samples. (c) 2021 Elsevier B.V. All rights reserved.
关键词	Ratiometric fluorescence sensor Cascade reaction Oxidase-mimics Ce4+ O-Phenlenediamine Ascorbic acid
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60818
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xia, Meng,Shi, Fengjin,Xia, Yinghui,et al. Ce4+-triggered cascade reaction for ratiometric fluorescence detection of alendronate[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2021,251.
APA	Xia, Meng,Shi, Fengjin,Xia, Yinghui,Sun, Jing,Zhao, Xian-En,&Zhu, Shuyun.(2021).Ce4+-triggered cascade reaction for ratiometric fluorescence detection of alendronate.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,251.
MLA	Xia, Meng,et al."Ce4+-triggered cascade reaction for ratiometric fluorescence detection of alendronate".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 251(2021).