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A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells
Wang, Peng; Sun, Liangyu; Wu, Jiang; Yang, Xiupei; Lin, Pengchen; Wang, Min
2021
发表期刊JOURNAL OF HAZARDOUS MATERIALS
卷号407
摘要Highly sensitive and selectivite detection of copper ions (Cu2+) and hydrogen sulfide (H2S) have become important research topics due to the potential harmful impacts of these chemicals to human health and the environment. In this study, we report the synthesis of a dual-functional peptide-based probe L (FITC-AhxSer-SerHis), designed to mimic a copper-sulfur metalloprotein, and capable of continuous detection of Cu2+ and S2- based on colorimetric and fluorescent methods. The new probe L displayed excellent turn off fluorescence response and good selectivity for Cu2+ ions via a modification of the tripeptide and fluorescein isothiocyanate group, and produced an obvious color change visible to the naked eye. Furthermore, as an excitable probe, the L-Cu complex could continuously detect S2- with high selectivity and sensitivity in 100% aqueous buffered solutions. The detection limits for fluorescence titration measurements, calculated using the equation 3 sigma/k, were 76.7 nM (Cu2+) and 27.2 nM (S2-), which were well below U.S. EPA safety levels. In addition, L could be cycled to alternately detect Cu2+ and S2-, thereby making it a promising reversible probe. Moreover, L was successfully applied to monitoring Cu2+ and S2- in live RKO cells through fluorescence imaging, exhibiting low cytotoxicity and good cell permeability.
关键词Peptide Probe Cu2+ S2- Aqueous medium Cell imaging
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/60820
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Wang, Peng,Sun, Liangyu,Wu, Jiang,et al. A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,407.
APA Wang, Peng,Sun, Liangyu,Wu, Jiang,Yang, Xiupei,Lin, Pengchen,&Wang, Min.(2021).A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells.JOURNAL OF HAZARDOUS MATERIALS,407.
MLA Wang, Peng,et al."A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells".JOURNAL OF HAZARDOUS MATERIALS 407(2021).
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