Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells

NWIPB OpenIR

	Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells
	Jin, Youcai; Luan, Guangxiang; Li, Ji; Wang, Honglun; Wang, Zhenhua; Bai, Bo
	2021
发表期刊	MOLECULAR MEDICINE REPORTS
卷号	23 期号:4
摘要	Malignant tumors of the central nervous system (CNS) are among the types of cancer with the poorest prognosis and glioma is the commonest primary CNS tumor. A mitochondrial DNA (mtDNA)-depleted cell line C6 rho 0 was generated from C6 glioma cells after long-term exposure to ethidium bromide and 2 ',3 '-dideoxycytidine in order to determine the effect of mtDNA damage on cell proliferation and pathological changes in glioma cells. Single cell clones were isolated and identified after 42 days of incubation. Repopulated cybrids were formed when the clonal C6 rho 0 cells were fused with rat platelets and no difference was observed in their growth in a selective medium without uridine and pyruvate compared with the growth of the parent C6 cells. Disruption of mtDNA resulted in changes in mitochondrial morphology, decreased cell proliferation, reduced intracellular reactive oxygen species and intracellular ATP, along with decreased mtDNA and mitochondrial membrane potential in C6 rho 0 cells compared with the C6 cells. Taken together, C6 rho 0 cells without mtDNA were established for the first time and their characteristics were compared with parent cells. This C6 rho 0 cell line could be used to explore the contribution of mitochondrial dysfunction and mtDNA mutations in the pathogenesis of glioma.
关键词	C6 glioma ρ 0 mitochondrial DNA cytoplasmic hybrids mitochondrial
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60827
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jin, Youcai,Luan, Guangxiang,Li, Ji,et al. Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells[J]. MOLECULAR MEDICINE REPORTS,2021,23(4).
APA	Jin, Youcai,Luan, Guangxiang,Li, Ji,Wang, Honglun,Wang, Zhenhua,&Bai, Bo.(2021).Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells.MOLECULAR MEDICINE REPORTS,23(4).
MLA	Jin, Youcai,et al."Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells".MOLECULAR MEDICINE REPORTS 23.4(2021).