Albumin-mediated alteration of plasma zinc speciation by fatty acids modulates blood clotting in type-2 diabetes

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	Albumin-mediated alteration of plasma zinc speciation by fatty acids modulates blood clotting in type-2 diabetes
	Sobczak, Amelie I. S.; Katundu, Kondwani G. H.; Phoenix, Fladia A.; Khazaipoul, Siavash; Yu, Ruitao; Lampiao, Fanuel; Stefanowicz, Fiona; Blindauer, Claudia A.; Pitt, Samantha J.; Smith, Terry K.; Ajjan, Ramzi A.; Stewart, Alan J.
	2021
发表期刊	CHEMICAL SCIENCE
卷号	12 期号:11
摘要	Zn2+ is an essential regulator of coagulation and is released from activated platelets. In plasma, the free Zn2+ concentration is fine-tuned through buffering by human serum albumin (HSA). Importantly, the ability of HSA to bind/buffer Zn2+ is compromised by co-transported non-esterified fatty acids (NEFAs). Given the role of Zn2+ in blood clot formation, we hypothesise that Zn2+ displacement from HSA by NEFAs in certain conditions (such as type 2 diabetes mellitus, T2DM) impacts on the cellular and protein arms of coagulation. To test this hypothesis, we assessed the extent to which increasing concentrations of a range of medium- and long-chain NEFAs reduced Zn2+-binding ability of HSA. Amongst the NEFAs tested, palmitate (16 : 0) and stearate (18 : 0) were the most effective at suppressing zinc-binding, whilst the mono-unsaturated palmitoleate (16 : 1c9) was markedly less effective. Assessment of platelet aggregation and fibrin clotting parameters in purified systems and in pooled plasma suggested that the HSA-mediated impact of the model NEFA myristate on zinc speciation intensified the effects of Zn2+ alone. The effects of elevated Zn2+ alone on fibrin clot density and fibre thickness in a purified protein system were mirrored in samples from T2DM patients, who have derranged NEFA metabolism. Crucially, T2DM individuals had increased total plasma NEFAs compared to controls, with the concentrations of key saturated (myristate, palmitate, stearate) and mono-unsaturated (oleate, cis-vaccenate) NEFAs positively correlating with clot density. Collectively, these data strongly support the concept that elevated NEFA levels contribute to altered coagulation in T2DM through dysregulation of plasma zinc speciation.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60832
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Sobczak, Amelie I. S.,Katundu, Kondwani G. H.,Phoenix, Fladia A.,et al. Albumin-mediated alteration of plasma zinc speciation by fatty acids modulates blood clotting in type-2 diabetes[J]. CHEMICAL SCIENCE,2021,12(11).
APA	Sobczak, Amelie I. S..,Katundu, Kondwani G. H..,Phoenix, Fladia A..,Khazaipoul, Siavash.,Yu, Ruitao.,...&Stewart, Alan J..(2021).Albumin-mediated alteration of plasma zinc speciation by fatty acids modulates blood clotting in type-2 diabetes.CHEMICAL SCIENCE,12(11).
MLA	Sobczak, Amelie I. S.,et al."Albumin-mediated alteration of plasma zinc speciation by fatty acids modulates blood clotting in type-2 diabetes".CHEMICAL SCIENCE 12.11(2021).