Dual-signal based immunoassay for colorimetric and photothermal detection of furazolidone

NWIPB OpenIR

	Dual-signal based immunoassay for colorimetric and photothermal detection of furazolidone
	Su, Lihong; Chen, Yaqian; Wang, Lulu; Zhang, Han; Sun, Jing; Wang, Jianlong; Zhang, Daohong
	2021
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	331
摘要	As a point-of-care testing method, lateral flow immunoassays are attracting extensive attentions for antibiotic residue detections; however, immunoassay techniques for qualitative and quantitative detections are limited by the poor sensitivity and requirement for cumbersome quantitative devices. To overcome these limitations, MnO2-Au, with an excellent carrying capacity of Au nanoparticles and high photothermal signals, was introduced and a dual-signal immunoassay using colorimetric/photothermal MnO2-Au signal probe was proposed for furazolidone (FZD) detection. The dual-signal immunoassay not only enables visual detection relying on color change, but also enables quantitative detection via converting conventional assay signals into heat signals using a thermal infrared imager recording. Based on the excellent light-to-heat conversion activity of the bifunctional MnO2-Au, the FZD metabolite of 3-amino-2-oxazolidinone (AOZ) can be qualitatively detected in virtue of colorimetric signals with a visual limit of 1 ng mL(-1) and quantitatively detected by photothermal signals with a detection limit of 0.43 ng mL(-1). The dual-signal immunoassay biosensor was well applied in food samples with acceptable recoveries of 80.6-106 %. The immunoassay that integrates colorimetric and photothermal analysis will hold a broader application area in real-time monitoring.
关键词	Furazolidone Immunoassay Dual-signal Food safety
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60834
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Su, Lihong,Chen, Yaqian,Wang, Lulu,et al. Dual-signal based immunoassay for colorimetric and photothermal detection of furazolidone[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,331.
APA	Su, Lihong.,Chen, Yaqian.,Wang, Lulu.,Zhang, Han.,Sun, Jing.,...&Zhang, Daohong.(2021).Dual-signal based immunoassay for colorimetric and photothermal detection of furazolidone.SENSORS AND ACTUATORS B-CHEMICAL,331.
MLA	Su, Lihong,et al."Dual-signal based immunoassay for colorimetric and photothermal detection of furazolidone".SENSORS AND ACTUATORS B-CHEMICAL 331(2021).