NWIPB OpenIR
Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol
Wang, Lulu; Zhang, Han; Su, Lihong; Yao, Xiaolin; Wang, Zonghan; Zhao, Man; Sun, Jing; Wang, Jianlong; Zhang, Daohong
2021
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号331
摘要Exploring a nanomaterial simultaneously possessing simple synthesis process and brighter colors as a novel label is urgently needed in the immunochromatography assay (ICA). Herein, in order to achieve high-sensitive detection of small molecule pollutants, an immunosensor is developed, where the mild resorcinol formaldehyde resin polymer (mRF, 380 nm) synthesized under mild conditions, was applied as a signal label. The mRF possesses good biocompatibility, various chemical groups, and is easy to bind biological molecules. The antibody was connected to the mRF through electrostatic adsorption to prepare immune probes that was further applied in ICA. In this work, clenbuterol (CL) was chosen as the analysis model to prove the performance of the mRF-based immune probe. Under best conditions, the detection sensitivity is 1 ng mL(-1), that is 4 times lower than that of as much sensitive as the traditional colloidal gold strip. In addition, this method was successfully applied to detect CL in food samples, with recovery rates between 96.7 % and 117.2 %. These results show that the mRF-based ICA is a reliable and sensitive detection tool for the CL residue. This work offers a new possibility for the application of RF and a new reference method for the analysis and detection of other small molecule pollutants.
关键词Immunochromatography assay Mild reaction condition Resorcinol formaldehyde resin Clenbuterol
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/60835
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Wang, Lulu,Zhang, Han,Su, Lihong,et al. Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,331.
APA Wang, Lulu.,Zhang, Han.,Su, Lihong.,Yao, Xiaolin.,Wang, Zonghan.,...&Zhang, Daohong.(2021).Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol.SENSORS AND ACTUATORS B-CHEMICAL,331.
MLA Wang, Lulu,et al."Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol".SENSORS AND ACTUATORS B-CHEMICAL 331(2021).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Lulu]的文章
[Zhang, Han]的文章
[Su, Lihong]的文章
百度学术
百度学术中相似的文章
[Wang, Lulu]的文章
[Zhang, Han]的文章
[Su, Lihong]的文章
必应学术
必应学术中相似的文章
[Wang, Lulu]的文章
[Zhang, Han]的文章
[Su, Lihong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。