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Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay
Liu, Chuang; Lei, Yuqing; Dang, Jun; Wang, Weidong; Zhang, Jing; Mei, Lijuan; Liu, Zenggen; Tao, Yanduo; Shao, Yun
2021
发表期刊JOURNAL OF SEPARATION SCIENCE
卷号44期号:7
摘要The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid chromatography under the guidance of an online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products.
关键词free radical inhibitors liquid chromatography medium-pressure chromatography Ribes himalense
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/60855
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Liu, Chuang,Lei, Yuqing,Dang, Jun,et al. Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay[J]. JOURNAL OF SEPARATION SCIENCE,2021,44(7).
APA Liu, Chuang.,Lei, Yuqing.,Dang, Jun.,Wang, Weidong.,Zhang, Jing.,...&Shao, Yun.(2021).Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay.JOURNAL OF SEPARATION SCIENCE,44(7).
MLA Liu, Chuang,et al."Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay".JOURNAL OF SEPARATION SCIENCE 44.7(2021).
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