Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay | |
Liu, Chuang; Lei, Yuqing; Dang, Jun; Wang, Weidong; Zhang, Jing; Mei, Lijuan; Liu, Zenggen; Tao, Yanduo; Shao, Yun | |
2021 | |
发表期刊 | JOURNAL OF SEPARATION SCIENCE |
卷号 | 44期号:7 |
摘要 | The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid chromatography under the guidance of an online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products. |
关键词 | free radical inhibitors liquid chromatography medium-pressure chromatography Ribes himalense |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60855 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Liu, Chuang,Lei, Yuqing,Dang, Jun,et al. Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay[J]. JOURNAL OF SEPARATION SCIENCE,2021,44(7). |
APA | Liu, Chuang.,Lei, Yuqing.,Dang, Jun.,Wang, Weidong.,Zhang, Jing.,...&Shao, Yun.(2021).Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay.JOURNAL OF SEPARATION SCIENCE,44(7). |
MLA | Liu, Chuang,et al."Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay".JOURNAL OF SEPARATION SCIENCE 44.7(2021). |
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