Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters

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	Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters
	Liu, Lingyuan; Zhu, Shuyun; Sun, Jing; Xia, Meng; Zhao, Xian'en; Xu, Guobao
	2021
发表期刊	CHINESE CHEMICAL LETTERS
卷号	32 期号:2
摘要	We design a ratiometric fluorescent sensing platform for bleomycin (BLM) by using proximity-dependent DNA-templated silver nanoclusters (DNA-AgNCs) probe. This ratiometric sensing system is constructed with DNA-AgNCs as single fluorophore. The proposed strategy is based on the two following facts: (1) a covert DNA can approach and transform the DNA-AgNCs with green emission (G-DNA-AgNCs) into red emission through hybridization reaction. (2) The specific cleavage of the convert DNA by BLM in the presence of Fe(II) inhibits the discoloration of G-DNA-AgNCs. Thus, benefiting from the specific recognition of BLM and unique properties of G-DNA-AgNCs, a highly-sensitive ratiometric sensor for BLM has been successfully developed. The detection limit is as low as 30 pmol/L. This label-free fluorescence probe possesses advantages of convenient synthetic process and low cost. Moreover, this ratiometric method has been applied to the detection of BLM in human serum samples, illustrating a promising tool for analysis of BLM in cancer therapy. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
关键词	Ratiometric nanosensor DNA-templated silver nanoclusters Fluorescence transformation Bleomycin DNA scission
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60859
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Lingyuan,Zhu, Shuyun,Sun, Jing,et al. Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters[J]. CHINESE CHEMICAL LETTERS,2021,32(2).
APA	Liu, Lingyuan,Zhu, Shuyun,Sun, Jing,Xia, Meng,Zhao, Xian'en,&Xu, Guobao.(2021).Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters.CHINESE CHEMICAL LETTERS,32(2).
MLA	Liu, Lingyuan,et al."Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters".CHINESE CHEMICAL LETTERS 32.2(2021).