HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent

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	HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent
	Yang, Xue; Liu, Yongling; Chen, Tao; Wang, Nana; Li, Hongmei; Zhao, Jingyang; Li, Yulin
	2021
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	59 期号:2
摘要	Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6 ''-acetate.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60862
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Xue,Liu, Yongling,Chen, Tao,et al. HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2021,59(2).
APA	Yang, Xue.,Liu, Yongling.,Chen, Tao.,Wang, Nana.,Li, Hongmei.,...&Li, Yulin.(2021).HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent.JOURNAL OF CHROMATOGRAPHIC SCIENCE,59(2).
MLA	Yang, Xue,et al."HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent".JOURNAL OF CHROMATOGRAPHIC SCIENCE 59.2(2021).